CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



157 



4. MgS04 0.2 g. 



5. NaCl 0.01 g. 



6. MnS04 0.01 g. 



7. Peptone 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Dissolve 3% of one of the added 

 nutrients. 



(3) Adjust the reaction to pH = 7.0. 

 Sterilization: Not specified. 



Use: Cultivation of acetone butyl alcohol 



organisms. 

 Added nutrients: The authors added 3% of 



any desired carbohydrate. 

 Reference: McCoy, Fred, Peterson and 



Hastings (1926 p. 460). 



SUBGROUP I-C. SECTION 8 



Incomplete or basal solutions containing 

 peptone as the sole organic nitrogen and 

 carbon source; employed with the addition 

 of a variety of materials. 

 Ai. No salts added. 

 Bi. Indicators employed. 

 Morishima's Basal Phenol Red China 



Blue Peptone Solution 566 



Robinson's Basal Azolitrain Peptone 



Solution 567 



B2. Indicator not employed. 

 Capaldi and Proskauer's Basal Pep- 

 tone Solution 568 



A2. Salts added. 



Bi. Only one salt added. 



Ci. Salts of only monovalent cations 



employed. 

 Di. Sodium salt added. 

 El. Indicator employed. 



Barsiekow's Basal Salt Peptone Solu- 

 tion 569 



Cunningham's Basal Fuchsin Pep- 

 tone Solution 570 



Harvey's Basal Neutral Red Peptone 



Solution 571 



Abba's Basal Phenolphthalein Pep- 

 tone Solution 572 



E2. Indicators not employed. 

 Mendel's Basal Peptone Solution... 573 

 Nicolle, Raphael and Debains' Basal 



Peptone Solution 574 



Robinson and Rettger's Basal Opsine 



Solution 575 



D2. Potassium salt added. 

 Elser and Huntoon's Basal Nahr- 

 stofT-Heyden Solution 576 



Clark and Lubs' Basal Phosphate 



Peptone Solution 577 



C2. Salts of divalent cations employed. 



Grimbert's Basal Peptone Solution. . 578 

 B2. More than one salt added. 

 Ci. Salts of only monovalent cations added. 

 Kendall, Walker and Day's Basal 



Peptone Salt Solution 579 



Berman and Rettger's Basal Peptone 



Salt Solution 580 



Co.* Salts of only mono and divalent 



cations added. 

 Di. Containing calcium salts. 

 Palladin's Basal Peptone Salt Solu- 

 tion 581 



Boekhout and Ott de Vries' Basal 



Peptone Salt Solution 582 



Will's Basal Peptone Salt Solution. . 583 

 D2. Not containing calcium salts. 

 Maassen's Basal Peptone Salt Solu- 

 tion 584 



Calmette, Massol and Breton's Basal 



Peptone Salt Solution 585 



Hansen's Basal Peptone Salt Solu- 

 tion 586 



C3. Salts of mono, di and trivalent cations 

 added. 

 Stoklasa and Vitek's Basal Peptone 



Salt Solution 587 



Vierling's Basal Peptone Salt Solu- 

 tion ., 588 



566. Morishima's Basal Phenol Red China 

 Blue Peptone Solution 



Constituents: 



1. Peptone solution 1000.0 cc. 



2. Phenol red (0.02% soln.) . . . 50.0 cc. 



3. China blue decolorized 



(1.0% soln.) 12.0 cc. 



Preparation : 



(1) Method of preparation of 1 not given. 

 Any fluid medium used to determine 

 fermentation may be used. 



(2) Prepare a 1.0% solution of China blue 

 in distilled water, and keeping the 

 water hot on a water bath, normal 

 NaOH solution is slowly added until 

 the blue color disappears and the 

 solution becomes brown. (About 

 3.5 cc. N/1 NaOH is required per 

 100.0 cc. of China blue.) Keep in 

 rubber stoppered flask. 



(3) Prepare a 0.02% solution of phenol red. 



* See C3. 



