158 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(4) Adjust (1) to desired pH. 



(5) Add 5.0 cc. of (3) and 1.2 cc. of (2) 

 to (4) for every 100.0 cc. of (4). 



(6) Add one of the added nutrients. 

 Sterilization: Autoclave at 10 pounds pres- 

 sure for 10 minutes. 



Use: To determine fermentation of sugars. 

 Pink color indicates alkali formation. 

 Acid indicated by first a bright green and 

 then changing to deep blue. 



Added nutrients: Author suggested the use 

 of any carbohydrate or fermentable ma- 

 terial. The carbohydrate solution may 

 be sterilized separately and added aseptic- 

 ally to sterile (5), or the sugar can be 

 added to sterilized (5) and the mixture 

 be heated a second time in the autoclave 

 at 10 pounds for 10 minutes. 



Reference: Morishima (1920 p. 43). 



567. Robinson's Basal Azolitmin Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. Azolitmin 0.5 g. 



Preparation : 



(1) Prepare 500.0 cc. of a 2.0% solution of 

 Witte' s peptone. 



(2) Adjust to a reaction of -f-0.6 to 

 phenolphthalein. 



(3) Prepare 500.0 cc. of a solution con- 

 taining 2.0% of an added nutrient 

 (sugar). 



(4) Mix sterile (2) and (3) in equal 

 amounts, and add 0.05% azolitmin. 



(5) Tube in sterile tubes. 

 Sterilization: Sterilize (2) in the autoclave. 



Filter (3) thru Berkefeld candles into 



sterile flasks. Incubate 24 hours to 



insure sterility. 

 Use : To determine fermentation by typhoid 



and paratyphoid bacilli. 

 Added nutrients and variants : 



(a) Robinson used 10.0 g. of any desired 

 sugar. 



(b) Harvey used 2.0 to 10.0 g. peptone, 

 50.0 cc. of litmus solution and 10.0 g. 

 of any desired carbon source or fer- 

 mentable material. 



(c) Harvey used 20.0 g. peptone, 50.0 cc. 

 of litmus solution and 10.0 g. of any 

 carbon source or fermentable ma- 

 terials per 850.0 cc. of distilled water. 



References: Robinson (1915 p. 407), Harvey 

 (1921-22 pp. 101, 109). 



568. Capaldi and Proskauer's Basal Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) (0.5 



or 2.0%) 5.0 or 20.0 g. 



Preparation: (1) Dissolve 2 and 0.1% of one 

 of the added nutrients in 1. 



Sterilization: Not specified. 



Use: To determine fermentation of sugars 

 by the colon-typhoid group. Different 

 investigators added various materials to 

 the basic solution, or a modification of it, 

 and used the media for a variety of 

 purposes. 



Added nutrients and variants: 



(a) The authors added 0.1% of one of 

 the following materials: 



glucose raffinose 



levulose dextrin 



mannose inulin 



galactose mannitol 



sorbose adonitol 



lactose sorbitol 



maltose isodulcitol 



melibiose erythritol 



trehalose dulcitol 

 They reported growth poor with a 

 M.L.D. = 1.0-f- cc. 



(b) Matzuschita used 10.0 g. Koch's 

 meat peptone per liter water as a 

 basic solution and added one of the 

 following materials or combinations: 



NaCl 0.5, 0.7 to 10.5%. 



NaCl 5.0 g. and glucose 0.5 to 50.0%. 



NaCl 5.0 g., glucose 2.0% and from 0.2 



to 15.0% Na.COs. Add the NajCOs 



to the neutral medium. 

 NaCl 5.0 g., glucose 2.0%, glycerol 



6.0%. 

 NaCl 5.0 g., glucose, 2.0% and from 0.1 



to 0.4% HCl. Add the HCl to the 



neutral medium. 

 No additions to the basic solution. 



These media were used to cultivate 



Clostridium butyricum, Bacillus 



oedematis maligni, Bacillus anthracis 



sym-ptomaiici, Bacillus sporogenes, 



Bacillus botulinus. 



(c) Revis did not specify the exact com- 

 position of the basic solution (pep- 



