CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



159 



t:)ne water) and added one of the 

 following materials: 



mucic acid (sodium salt) 1-0% 



saccharic acid (potassium salt). . 1.0% 

 gluconic acid (potassium salt)... 0.5% 

 The media were used to study the 

 fermentation of acids by the colon 

 groip. The author reported that 

 with 1.0% of the acid sodium salt of 

 mucic acid no gas was produced with 

 B. coll or B. lactis aerogenes, but 

 slightly more acid was produced. 

 With the normal sodium salt and in 

 the presence of Mg(0H)2 acid and 

 gas were produced by B. coli and B. 

 lactis aerogenes. 



Same thing was true with saccharic 

 acid. (Acid and gas produced when 

 medium was alkaline.) Gluconic 

 acid was attacked when present as 

 the potassium salt or when alkalinity 

 was increased to 1.0 cc. N/1 NaOH 

 per 100.0 cc, or when the acid itself 

 was employed. 



(d) Davis and Ferry cultivated Bad. 

 diphtheriae and studied toxin pro- 

 duction using 20.0 g. of peptone in the 

 basic solution and adding one of the 

 following materials: 



glycocoll 0.75 g 



leucine 30.0 g 



histidine dichloride 0.5 g 



glutaminic acid hydrochloride. 2.5 g 



tyrosine 20.0 g 



cystine 0.5 g 



tryptophane 0.6 g 



sodium asparaginate 1.5 g 



creatine 0.2g + creatinine 0.15g. 

 xanthin 0.05 g. + hypoxanthine 0.05 g 

 glucose amine hydrochloride . . 2.0 g 



(e) Holm and Sherman used 10.0 g. of 

 peptone per liter in their basic solu- 

 tion and added one of the following 

 salts in sufficient quantities to make 

 the medium 0.2 molar for each salt. 



NaCl Na citrate 



NaXOs Na fluoride 



NaHP04 KCl 



Nal CaCl2 



Na2S04 MgClo 



Na lactate FeClj 



Na o.xalate NH4CI 



Na acetate 

 In case of sodium lactate, NaOH is 



added to lactic acid until a pH = 7.0 

 is reached. In case of the phos- 

 phates, mono and di-sodium phos- 

 phates are mixed in the proper 

 proportions to give a pH of approxi- 

 mately 7.0. NaCl is used also in 

 0.4M and MgCU in O.IM concentra- 

 tions. The media were used to show 

 the effect of salts on the growth of 

 B. coli. The authors reported that 

 generally the order of growth accele- 

 ration of B. coli by these salts was 

 approximately the same as their 

 order in the lyotropic series, 

 (f) Hotchkiss used 10.0 g. peptone per 

 liter in the basic solution and added 

 a number of salts to study the effect 

 of various cations on a communis 

 type of B. coli. He used the follow- 

 ing salts and reported that the 

 concentrations here indicated stimu- 

 lated growth. NaCl, KCl, CaCU and 

 MgCl2 may be added to the peptone 

 before sterilization. Other salt solu- 

 tions were made up and stored until 

 sterile and added under aseptic 

 conditions to the sterile peptone 

 solution. pH for all solutions except 

 NH4CI were from 6.6 to 7.0. NH4CI 

 solution pH = 6.0 to 6.4. 

 NaCI 0.25 molar concentration in the 



media. 

 KCl 0.25 molar concentration in the 



media. 

 NH4CI 0.25 molar concentration in the 



media. 

 CaCU 0.05 molar concentration in the 



media. 

 MgClo 0.05 molar concentration in the 



media. 

 SrCl2 0.025 molar concentration in the 



media. 

 BaCl2 0.05 molar concentration in the 



media. 

 TiCl2 0.0005 molar concentration in 



the media. 

 NiCl2 0.0001 to 0.00005 molar concen- 

 tration in the media. 

 PbCla 0.0005 to 0.00005 molar concen- 

 tration in the media. 

 SnCl2 0.00005 to 0.000005 molar con- 

 centration in the media. 

 ZnCl2 0.00005 to 0.00001 molar con- 

 centration in the media. 



