160 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



CeCl2 0.00001 molar concentration in 



the media. 

 HgCl2 0.000001 molar concentration in 

 the media. 

 References: Capaldi and Proskauer (1894 

 p. 467), Matzuschita (1902 p. 286), Revis 

 (1912 p. 427), Davis and Ferry (1919 

 p. 235), Holm and Sherman (1921 p. 515), 

 Hotchkiss (1923 pp. 142-161). 



569. Barsiekow's Basal Salt Peptone 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Lackmus tincture 100.0 g. 



3. Peptone (1.0%) 10.0 g. 



4. NaCl (0.5%) 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 1.0% 



of one of the added nutrients in 1. 



Sterilization: Not specified. 



Use: Differentiation of colon typhoid 

 group. Colon forms turned the medium 

 red, typhoid and alkaligenes types blue. 



Added nutrients and variants: 



(a) The author added 1.0% glucose or 

 1.0% lactose. 



(b) Harvey used a similar basic solution 

 and prepared it as follows : 



(1) Dissolve 10.0 g. peptone, 5.0 g. 

 NaCl in a liter of water by 

 steaming. 



(2) Make faintly alkaline to litmus by 

 the addition of sodium carbonate. 



(3) Filter. 



(4) Sterilize. 



(5) Place solid commercial litmus in a 

 flask and pour 96% alcohol over it. 



(6) Close (5) with a rubber stopper. 



(7) Keep in an incubator at 30°C. for 

 2 days, shaking occasionally. 



(8) Pour off the alcohol and add 

 fresh. 



(9) Continue the process until the 

 alcohol extracts no more color. 



(10) Filter off the alcohol. 



(11) Dry the residue. 



(12) Dissolve (11) in distilled water to 

 saturation. 



(13) Add sufficient 1-1000 sulphuric 

 acid to produce the slightest color. 



(14) Dissolve 10.0 g. of any desired 

 carbon source of fermentable ma- 

 terial in 50.0 cc. of (13). 



(15) Add (14) to (4). 



(16) Filter into test tubes. 



(17) Sterilize for 2 days at lOctc. and 

 allow the temperature p reach 

 120°C. on the third day (Ind then 

 extinguish the flame. 



References; Barsiekow (1901 pp. 823-825), 

 Harvey (1921-22 p. 108). 



570. Cunningham's Basal Fuchsin Peptone 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. Andrades indicator (1.0%). 10.0 cc. 

 Preparation: 



(1) Dissolve 2 and 3 in 1 by autoclaving 

 to 2 atmospheres pressure. 



(2) Filter thru paper. 



(3) Dissolve 0.5% of one of the added 

 nutrients and 1.0% of Andrades 

 indicator in (2). 



(4) Tube with Dunham's fermentation 

 tubes. 



(5) Stand the tubes in a water bath and 

 heat to boiling. 



(6) Plug. 



Sterilization: Sterilize in the autoclave at 

 22.5 pounds pressure. (Do not exceed 

 22.5 pounds pressure.) 



Use: To observe fermentation. 



Added nutrients: The author added 0.5% 

 of any desired sugar. 



Reference: Cunningham (1924 p. 18). 



571. Harvey's Basal Neutral Red Peptone 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. Peptone 10.0 g. 



4. 1.0% neutral red 2.5 cc. 



Preparation : 



(1) Dissolve 2 and 3 in 1 by steaming. 



(2) Filter. 



(3) Add freshly prepared 1.0% neutral 

 red 2.5 cc. 



(4) Prepare a 10.0% solution of one of 

 the added nutrients. 



(5) Add sufficient amount of sterile (4) 

 to sterile (3) so that the concentra- 

 tion of the added nutrient is 1.0%. 



(6) Distribute in test tubes containing 

 fermentation tubes. 



