CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



161 



Sterilization: Sterilize (3) in the autoclave. 

 Sterilize (4) at 105°C. for 10 minutes. 

 Sterilize the tubes at 105°C. for 10 

 minutes or at 100° on each of 2 successive 

 days for 20 minutes. 



Use: To observe fermentation by bacteria. 



Added nutrients: The author did not 

 specify the sugars used. One per cent of 

 any desired carbon source may be em- 

 ployed. 



Reference: Harvey (1921-22 p. 108). 



572. Abba's Basal Phenolphthalein Peptone 



Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Peptone 100.0 g. 



3. NaCl 50.0 g. 



4. Phenolphthalein (1.0% alco- 

 holic soln.) 0.5 cc. 



Preparation : 



(1) Dissolve 2, 3 and 200.0 g. of one of the 

 added nutrients in 1. 



(2) Boil in the steamer for 30 minutes at 

 100°C. 



(3) Filter. 



(4) Distribute into 100.0 cc. lots into 

 sterile containers. 



(5) After sterilization add 0.5 cc. of 

 phenolphthalein solution and Na2C03 

 solution until the solution is perma- 

 nently red (see use). 



Sterilization: Not specified. 



Use: Isolation of Bacillus coli communis 

 from water. About 1 liter of the water 

 under investigation is added to each flask 

 of medium. Bacillus coli communis 

 decolorized the solution after from 12 to 

 24 hours (using glucose). Using lactose 

 B. coli caused decolorization after about 

 8 to 24 hours. Typhoid bacilli require a 

 greater length of time. 



Added nutrients : The author added 200.0 g. 

 of lactose or glucose. 



Reference: Abba (1896 p. 13), (1896 p. 224), 

 (1895 ^176). 



573. Mendel's Basal Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. NaCl 5.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 1.0% of one of the added nu- 

 trients (see sterilization). 



Sterilization: Sterilize for 30 minutes on 

 each of 2 successive days. On the third 

 day add 1.0% of one of the added nutri- 

 ents, and sterilize for 15 minutes. 



Use: To study decomposition of sugars. 

 Other investigators used variants of the 

 medium to cultivate sludge forms and to 

 study proteolysis. 



Added nutrients and variants: 



(a) Mendel added 1.0%, of one of the 

 following: 



glucose lactose 



sucrose maltose 



(b) Rettger, Berman and Sturges studied 

 proteolysis used 5.0 g. NaCl and 2.5, 

 5.0 or 20.0 g. of Witte's peptone in 

 the basic solution and adding the 

 following materials individually or in 

 combination: 



glucose 10.0 g. 



beef extract 5.0 g. 



(NH4)2S04 2.5 g. 



(c) Brussoff used 10.0 g. NaCl in the 

 basic solution. He used the media 

 to cultivate Ferribacterium duplex, 

 and other iron bacteria from sludge. 

 He added one of the following ma- 

 terials to the basic solution. 



Iron ammonium citrate 0.5 g. 



K,C03 1.0 g. 



K2SO4 1.0 g. 



Potassium acetate 1.0 g. 



Potassium citrate 1.0 g. 



References: Mendel (1911 p. 297), Brussoff 

 (1918 pp. 195, 208) , Rettger, Burman and 

 Sturges (1916 p. 20-22). 



574. Nicolle, Raphael and Debains' Basal 

 Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Defresne) 1.0 g. 



3. Soda (normal) 20.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and one of the added 

 nutrients in 1. 



(2) Distribute in test tubes containing 

 fermentation tubes. 



Sterilization: Sterilize at 110° for 15 



minutes. 

 Use: To study the fermentation of sugars 



by typhoid and paratyphoid bacilli. 



