162 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Authors report that glucose may or may 



not be fermented, lactose never. 

 Added nutrients: Authors added 1.0% of 



glucose or lactose. 

 Reference: Nicolle, Raphael and Debains 



(1917 p. 379). 



575. Robinson and Rettger's Basal Opsine 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Opsine 10.0 g. 



3. NaCl 5.0 g. 



4. Glycerol 50.0 g. 



Preparation : 



(1) Dissolve 2, 3 and one of the added 

 nutrients in 1 by heating. 



(2) Adjust the reaction — may be used 

 either slightly acid or basic to litmus. 



(3) Boil over a flame a few minutes. 



(4) Filter and tube. 



Sterilization: Sterilize at 12 pounds extra 



pressure for 15 minutes. 

 Use: General culture medium. Opsine is 



a protein free trypsin, erepsin and pepsin 



digest of certain proteins. The medium 



supported the growth of some pathogenic 



forms. 

 Added nutrients: The authors added 5.0% 



glycerol or 1.0% glucose. 

 Reference: Robinson and Rettger (1918 



p. 212). 



576. Elser and Huntoon's Basal Nahrstoflf- 



Heyden Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Nahrstoff Heyden. . . . 10.0 g. 



3. NaCl 5.0 g. 



4. Litmus (Merck's 



soln) 5.0 to 7.5 cc. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 5.0 to 7.5 cc. of a watery solution 

 of Merck's highly sensitized litmus. 



(3) Prepare a 10.0% solution of one of 

 the added nutrients in distilled water. 



(4) Mix sterile (2) and sterile (3). 



(5) Tube in sterile tubes. 



(6) Incubate for 3 days to detect acci- 

 dental contamination. 



Sterilization: Sterilize (2) in the usual 

 manner, method not given. Sterilize 

 (3) by heating at 100°C. for 10 minutes. 



Use: To study fermentation by meningo- 

 cocci. 

 Added nutrients: The author added 10.0 g. 

 of one of the following: 



glucose sucrose 



galactose mannitol 



levulose dulcitol 



lactose inulin 



maltose dextrin 



Reference: Elser and Huntoon (1909 

 p. 404). 



577. Clark and Lubs' Basal Phosphate 

 Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte) 5.0 g. 



3. K2HPO4 (c.p.) 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and one of the added 

 nutrients in 800.0 cc. distilled water 

 by heating for 20 minutes over a 

 steam bath. Stir occasionally. 



(2) Filter thru a Schleicher and SchuU 

 No. 588 folded filter. 



(3) Cool to 20°C. and make up e.xactly 

 to 1000.0 cc. 



(4) Tube in 10.0 cc. quantities in clean 

 sterile test tubes. 



Sterilization: Sterilize by the intermittent 



method. 

 Use: Used to study fermentation of sugars. 

 Added nutrients and variants: 



(a) Clark and Lubs added 5.0 g. c.p. 

 glucose. 



(b) Levine added 5.0 g. of one of the 

 following materials: 



fructose raffinose 



galactose mannitol 



maltose glycerol 



lactose salicin 



sucrose dextrin 



The media were sterilized in the 

 autoclave. 



(c) Rogers, Clark and Evans used 10.0 g. 

 Witte's peptone instead of 5.0 g. and 

 added 10.0 g. of one of the following: 

 glucose inulin 

 lactose mannitol 

 sucrose dulcitol 

 raffinose adonitol 

 melibiose glycerol 

 arabinose 



The media was tubed in 10.0 cc. 



