CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



163 



quantities and sterilized by the inter- 

 mittent method in flowing steam. 



(d) Levine used Digestive Ferments 

 Co. peptone instead of Witte's, and 

 added 5.0 g. of glucose. The medium 

 was sterilized in the autoclave. 



(e) Burton and Rettger added 5.0 g. 

 glucose to the basic solution. The 

 reaction was adjusted to neutral to 

 litmus (pH = 7.0 or +1.0). Method of 

 sterilization not specified. 



(f) Kligler used 10.0 g. of peptone in- 

 stead of 5.0 g. Witte's peptone, added 

 5.0 g. NaCl, 1.0 g. of glucose and 

 added any organic test material in 

 any desired quantity. The ma- 

 terials used were added to test their 

 antiseptic ability. Sterilize the basic 

 solution with the glucose in the auto- 

 clave. Then add one of the ma- 

 terials under aseptic conditions. 



(g) Winslow, Rothberg and Parsons 

 added 27.0 cc. of a 0.04% alcoholic 

 solution of brom cresol purple and 

 5.0 g. of any carbon source or fer- 

 mentable material. The basic solu- 

 tion was adjusted to pH = 7.4, brom 

 cresol purple added and autoclaved 

 at 15 pounds pressure for 15 minutes. 

 The sterile added nutrient was added 

 under aseptic conditions. 



(h) Ayers, Rupp and Mudge used 40.0 g. 



of Bacto peptone instead of 5.0 g. 



Witte's peptone and added 2.0 g. 



glucose. Method of sterilization not 



specified, 

 (i) Cunningham autoclaved the K2HPO4, 



peptone and water to 30 pounds 



pressure, filtered, made up to 



1000.0 cc, distributed in 5.0 cc. 



quantities and autoclaved at 22.5 



pounds pressure, 

 (j) Wilson and Blair made up the basic 



solution with 5.0 g. glucose in double 



strength. They used the medium to 



enrich streptococci in water analyses. 



Method of sterilization not given. 



An equal volume of water under 



investigation was added to the 



medium. 



References: Clark and Lubs (1915 p. 169), 



Levine (1916 pp. 160, 161), Committee 



A. P. H. A. (1917 p. 107), Rogers, Clark 



and Lubs (1918 p. 234), Burton and 



Rettger (1917 p. 165), Kligler (1918 p. 467), 

 Tanner (1919 p. 47), Winslow, Rothberg 

 and Parsons (1920 p. 151), Levine (1921 

 p. 117), AyeTs, Rupp and Mudge (1921 

 p. 258), Giltner (1921 p. 383), Cunning- 

 ham (1924 p. 88), Wilson and Blair (1925 

 p. 112), Committee A. P. H. A. (1925 

 p. 111). 



578. Grimbert's Basal Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (dry) 20.0 g. 



3. CaCOs 

 Preparation : 



(1) Dissolve 2 and one of the added nu- 

 trients in 1. 



(2) Add a sufficient quantity of CaCOa. 

 Sterilization: Not specified. 



Use: Determine fermentation of Fried- 

 lander's pneumobacillus. 

 Added nutrients and variants: 



(a) The author added 30.0 g. of any 

 fermentable sugar. 



(b) Pottevin used 10.0 g. peptone, 12.0 g. 

 of CaCOa in the basic solution to 

 study the lactic fermentation by 

 lactic acid forming organisms. He 

 added one of the following: 



lactose 8.86 g 



sucrose 10.1 g 



maltose 11.4 g 



glucose 10.0 g 



Invert sugar 11.2 g 



galactose 9.2 g 



mannose 8.96 g 



mannitol 10.0 g 



dulcitol 10.0 g 



glycerol 10.0 g 



malic acid 10.0 g 



Reference: Grimbert (1895 p. 843), Pot- 

 tevin (1898 p. 54). 



579. Kendall, Walker and Day's Basal 



Peptone Salt Solution 



Constituents: 



1. Redistilled water 1000.0 cc. 



2. Peptone (Fairchild) 5.0 g. 



3. Na2HP04 2.0 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Extract 5.0 g. of Fairchild's peptone 

 for 2 weeks with ether, 2 weeks with 

 alcohol, 2 weeks with acetone and 10 



