164 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



days with petroleum ether, respec- 

 tively, in a Soxhlet extractor, the 

 successive extractions occurring at 

 intervals of about 12 minutes. These 

 extractions were continued for 6 

 hours a day for 6 days per week. 



(2) Dissolve the residue from (1), 3, 4 

 and one of the added nutrients in 1. 



(3) Distribute in 100.0 cc. lots. 

 Sterilization: Not specified. 



Use: To study lipolytic and proteolytic 

 activity of tubercle bacilli. To deter- 

 mine lipolytic activity, the authors placed 

 1.0 cc. of the bacteria free filtrate of the 

 culture in freshly boiled dist. H2O, then 

 added 0.25 cc. ethylbutyrate and 0.5 cc. 

 of toluene and incubated at 37°C. The 

 acid increase expressed in terms of X/50 

 NaOH was a measure of the lipolytic 

 activity. 



Added nutrients and variants: 



(a) The authors added 10.0 g. glucose or 

 20.0 g. glycerol. 



(b) Kendall, Day and Walker used 15.0 g. 

 of Fairchild's peptone (extracted) 

 and 3.0 g. NaoHPO^ in the basic solu- 

 tion. They added 10.0 g. glucose or 

 30.0 g. glycerol. The solution was 

 used to study the metabolism of 

 tubercle bacilli. Used a alizarin, 

 neutral red and phenolphthalein to 

 study changes in reaction, and Ziehl- 

 Neelsen stain for staining. 



(c) The authors used the same basic solu- 

 tion without extracting the peptone, 

 adding 10.0 g. glucose or 30.0 g. 

 glycerol. 



References: Kendall, Walker and Day 

 (1914 p. 463), Kendall, Day and Walker 

 (1914 p. 424), 



580. Berman and Rettger's Basal Peptone 

 Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (Witte) 2.5 or 5.0 g. 



3. NaCl 5.0 g. 



4. K2HPO4 5.0 to 8.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the combinations listed 

 under added nutrients to (1). 



Sterilization: Not specified. 



Use: To study nitrogen metabolism of 



bacteria. In presence of carbohydrates, 

 buffers aided nitrogen metabolism. 

 Added nutrients : One of the following com- 

 binations were added: 



(a) glucose, 2.0 to 10.0 g. 



(b) lactose, 2.0 to 10.0 g. 



(c) glucose, 2.0 to 10.0 g. + beef extract 

 2.5 g. 



(d) lactose, 2.0 to 10.0 g. + beef e.xtract 

 2.5 g. 



Reference: Berman and Rettger (1918 

 p. 392). 



581. Palladin's Basal Peptone Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Potassium phosphate 3.0 g. 



4. MgS04 1.0 g. 



5. CaCl, 1.0 g. 



6. FeCl. trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the added nutrients so 

 that the carbohydrate is present in 

 fourth normal concentration. 



(3) Distribute in thin layers in Erlen- 

 meyer flasks. 



Sterilization: Not specified. 



Use: To study fermentation by Cloro- 



thccium saccharophilum. 



Added nutrients : One of the following were 



added in fourth normal concentration: 



sucrose glucose 



mannitol raffinose 



Reference: Palladin (1903-04 p. 146). 



582. Boekhout and Ott de Vries' Basal 



Peptone Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (2.0%) 20.0 g. 



3. Bicalcium phosphate 



(0.2%) 2.0 g. 



4. CaCli (concentrated soln.). 5 drops 



5. NaCl (0.5%) 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and one 



of the added nutrients in 1 . 

 Sterilization: Not specified. 

 Use: Cultivation of organisms found in 



cheese. 

 Added nutrients: The authors added 0.5% 



of glucose or galactose. 



