CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



165 



Reference: Boekhout and Ott de Vries 

 (1918 p. 134). 



583. Will's Basal Peptone Salt Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. CaHP04 0.5 g. 



3. KH2PO4 4.55 g. 



4. Peptone (Witte) 20.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the added nutrients. 

 Sterilization: Not specified. 



Use: Cultivation of non-spore forming 



yeast. 

 Added nutrients and variants: 



(a) The author added one of the 

 following: 



sucrose 5.0% 



organic acid, carbohydrate or alcohol 



(b) Grosbiisch added one of the fol- 

 lowing: 



alcohol 5.0% 



formic acid 0.1% 



acetic acid 0.2% 



tartaric acid . .- 0.3% 



lactic acid 4.0% 



citric acid 8.0% 



malic acid 10.0% 



succinic acid 11.0% 



The amounts of acids given was the 

 largest amount at which Torida 

 rubefaciens developed. 

 Reference: Will (1908 p. 387), (1912 p. 3), 

 Grosbiisch (1920 p. 311). 



584. Maassen's Basal Peptone Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. KH2PO4 1-5 g. 



4. NaCl 1.0 g. 



5. MgS04 0.3 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 800.0 cc. of 1. 



(2) The reaction of (1) is acid. 



(3) Dissolve one tenth the equivalent 

 weight of one of the added nutrients 

 in 200.0 cc. of 1. 



(4) Add (3) to (1). 



(5) Neutralize and make slightly alkaline 

 to litmus. 



Sterilization: Not specified. 



Use: To study the utilization of organic 



acids by organisms. The author used 51 

 organisms and reported that 41 organisms 

 developed in the presence of malic acid 

 and 38 in the presence of citric and 

 fumaric acids. These acids allowed the 

 development of the greatest number of 

 organisms. 

 Added nutrients: One tenth equivalent 

 weight of one of the following acids was 

 added: 

 formic acid malonic acid 



acetic acid tartaric acid 



propionic acid fumaric acid 



glycolic acid malic acid 



lactic acid "trikarballysaure" 



butyric acid citric acid 



glyceric acid aconitic acid 



"Oralsaure" mucic acid 



malic acid quinic acid 



succinic acid mandelic acid 



Reference: Maassen (1895-6 p. 341). 



585. Calmette, Massol and Breton's Basal 



Peptone Salt Solution 

 Constituents: 



1. Water lOOO.O cc. 



2. NaaCOa 1.0 g. 



3. FeS04 0.04 g. 



4. MgS04 0.05 g. 



5. K0HPO4 1.05 g. 



6. NaCl 8.5 g. 



7. Peptone 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add one of the combinations given 

 under added nutrients to (1). 



Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. The 

 authors reported the best growth in the 

 presence of both glucose and glycerol. 



Added nutrients: One of the following 

 was added: 



(a) glycerol 40.0 g. 



(b) glucose 10.0 g. 



(c) glycerol 40.0 g. + glucose 10.0 g. 

 Reference: Calmette, Massol and Breton 



(1909 p. 580). 



586. Hansen's Basal Peptone Salt Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Peptone 1.0 g. 



3. KH2PO4 3.0 g. 



4. MgS04 2.0 or 5.0 g. 



