166 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation :(1) Dissolve 2, 3, 4 and one of 



the added nutrients in 1. (Use 5.0 g. 



MgS04 using maltose and 2.0 g. with 



glucose.) 

 Sterilization: Not specified. 

 Use: Cultivation of Saccharomyces cere- 



visiae, Saccharomyces Pastorianus and a 



wine yeast. 

 Added nutrients and variants: 



(a) The author added 5.0 g. of maltose 

 or glucose. 



(b) Stormer cultivated the causative 

 organism of water rust in flax (Plec- 

 tridium pectinovorum) using 5.0 g. 

 KH2PO4, 2.5 g. MgS04 and 5.0 g. of 

 peptone in the basic solution. One 

 of the following materials was added 



starch 5.0 to 10.0 g 



glucose 5.0 to 10.0 g 



arabinose 5.0 to 10.0 g 



dextrin 5.0 to 10.0 g 



galactose 5.0 to 10.0 g 



pectin substances 2.5 to 5.0 g 



All the solutions were sterilized in 

 the autoclave except the one con- 

 taining pectin. Sterilize this solu- 

 tion at 100°C. Incubate under anae- 

 robic conditions, using pyrogallic 

 acid and KOH. 



(c) Buromsky studied the effect of 

 organic acids upon the growth of 

 yeast, Saccharomyces ellipsoid, and 

 Saccharomyces pastorian. He used 

 0.5 g. MgS04, 1.0 g. KH2PO and 

 10.0 g. peptone in the basic solution 



• and added one of the following: 



citric acid 10% 



tartaric acid 10% 



malic acid 10% 



quinic acid 0.5% 



succinic acid 10% 



glycerol 1-0% 



mannitol 1-0% 



He reported no growth using tartaric 

 acid. Citric acid also inhibited most 

 yeast. 

 References: Hansen (1899 p. 6), Stormer 

 (1904 p. 177), Buromsky (1914 p. 532). 



587. Stoklasa and Vitek's Basal Peptone 

 Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Na2HP04 0.25 g. 



3. K2SO4 0.2 g 



4. NaaCOa 0.25 g 



5. FeP04 0.2 g 



6. MgCl2 0.1 g 



7. CaCl2 0.05 g 



8. Peptone 0.1 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Distribute in 100.0 9,0. lots. 



(3) Add one of the added nutrients to 

 each flask . (Neutralize and make the 

 acids slightly alkaline by the addi- 

 tion of NazCOs). 



(4) Add 2.0 g. CaCOs to each flask. 

 Sterilization: Method not given. 



Use: To study nitrogen assimilation by 

 Bacillus megatherium (Alinit bacteria). 

 Authors reported that organisms showed 

 greater development in medium contain- 

 ing organic acids as a source of carbon 

 than d-glucose. Greatest development 

 took place with saccharose, 1-arabinose 

 and l-.xylose. No growth took place using 

 d-laevulose or d-galactose. Lactic acid 

 is the best suited acid for B. megatherium 

 development. 

 Added nutrients: The authors added 10.0% 

 of one of the following: 



d-glucose maltose 



d-laevulose tartaric acid 



d-galactose citric acid 



d-xylose malic acid 



1-arabinose lactic acid 



saccharose butyric acid 



Reference: Stoklasa and Vitek (1901 



p. 268). 

 588. Vierling's Basal Peptone Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 1-0 g- 



3. CaCU 01 g- 



4. MgS04 0.1 g. 



5. FeCl3 trace 



6. NaCl trace 



7. Peptone 10-0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add one of the combinations given 

 under added nutrients. 



(3) Distribute in 5.0 cc. quantities in 

 tubes. 



Sterilization: Not specified. 



Use: To study nitrate reduction by myco- 



