CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



167 



bacteria. To test for nitrite production 

 add a 2.0% acidified Kt solution to the 

 tube. A blue color will develop in the 

 presence of nitrites. The presence of 

 nitrite may be detected also by dissolving 

 0.1 g. napthylamine in 150.0 g. dilute 

 acetic acid, and 0.5 g. of sulfanilic acid 

 in 150.0 g. dilute acetic acid. Mix 1.0 

 CO. of each solution and heat to boiling. 

 Add 1.0 CO. of the mixture to the culture. 

 If nitrite be present a red color will be 

 found. 

 Added nutrients and variants: 



(a) Starch 10.0 g. + KNO3 5.0 g. 



(b) Peptone 20.0 g. in the basic solution 

 and added 10.0 g. of starch. This 

 medium was used to study amylase 

 production by mycobacteria. No re- 

 ducing sugars were found to be present 

 using Fehling's solution for the test. 



(c) Glucose 10.0 g. This solution was 

 used to study catalase production by 

 mycobacteria. Catalase was pro- 

 duced. A 14 day culture quickly 

 decomposed 1.0 cc. of a 15.0 % H2O2 

 solution, of o.xygen with the libera- 

 of oxygen. 



Reference: Vierling (1920 pp. 201, 204, 

 207). 



SUBGROUP I-C. SECTION 9 



Complete or basal liquid media contain- 

 ing peptone or similar digests as sole sources 

 of nitrogen; additional organic carbon 

 supplied. 



Ai.* Carbohydrates present. 

 Bi. Carbohydrate only added. 

 Ci. Monosaccharides added. 

 Di. Levulose added. 



Miiller, Thurgau and Osterwalder's 



Levulose Peptone Solution 589 



D2. Glucose added. 



El. Containing phosphates. 



Fi. Containing salts of monovalent cations 



only. 



Kligler's Glucose Peptone Solution. . 590 



Lohnis' Glucose Peptone Solution... 591 

 F2. Containing salts of mono and divalent 



cations. 



Mueller's Glucose Peptone Salt Solu- 

 tion 592 



Schukow's Basal Glucose Peptone 

 Salt Solution 593 



Henneberg's Glucose Peptone Salt 



Solution 594 



Ej. Not containing phosphates. 

 Fi. Salts of monovalent cations only added. 

 Harvey's Glucose Peptone Solution. . 595 

 Eijkman's Glucose Peptone Solution. 596 

 Matzuschita's Glucose Peptone Solu- 

 tion 597 



F2. Salts of divalent cations with or without 

 other cations added. 

 Gosio's Glucose Peptone Solution... 598 



Sears' Glucose Peptone Solution 599 



Ringer's Solution Broth (Park, Wil- 

 liams and Krumwiede) 600 



Ci.* Disaccharides added. 

 Di. Lactose employed. 

 El. Inorganic salts not added. 

 Grimbert's Lactose Peptone Solu- 

 tion (Robin) 601 



Levine's Lactose Peptone Solution.. 602 

 Wagner and Monfort's Lactose Pep- 

 tone Solution 603 



Baginsky's Lactose Peptone Solution. 604 

 E2. Inorganic salts added. 

 Fi. Indicators added. 

 Robin's Lactose Peptone Solution. . . 605 

 Klein's Lactose Peptone Solution. . . . 606 

 Harvey's Lactose Peptone Solution. . 607 

 Olszewski and Kohler's Lactose Pep- 

 tone Solution 608 



Bacto Neutral Red Medium (Dehy- 

 drated) 609 



F2. No indicators added. 



Fischer and Andersen's Lactose Pep- 

 tone Solution 610 



Baginsky's Lactose Peptone Salt 



Solution 611 



D2. Sucrose employed. 



El. Containing salts of monovalent cations 

 only. 

 Bendick's Phenolphthalein Sucrose 



Solution 612 



Smith's Sucrose Peptone Solution 



(Owen) 613 



Briaudat's Sucrose Peptone Solution. 614 

 Bokorny's Sucrose Peptone Solution. 615 

 E2. Containing salts of mono and divalent 

 cations. 



Mutchler's Sucrose Peptone Solution. 616 

 Waksman's Sucrose Peptone Solution. 617 



Zikes' Sucrose Peptone Solution 618 



Behrens' Basal Sucro.se Peptone Solu- 

 tion 619 



* See ne.xt page for A2 and B2. 



*See C3 next page. 



