CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



169 



591. Lohnis' Glucose Peptone Solution 



Constituents: 



1. Water tap 1000.0 cc. 



2. Glucose (4.0%) 40.0 g. 



3. Peptone (0.1%) 1.0 g. 



4. K2HPO4 (0.05%) 0.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of aerobacter and amylo- 

 bacter. Tiie medium was inoculated 

 with 10.0% soil. Similar media were used 

 to study the fermentation of glucose, for 

 methyl red test. 



Variants : 



(a) Rogers, Clark and Evans prepared 

 the medium as follows: 



(1) Dissolve 10.0 g. glucose, Merck's 

 highest qi\ality, 10.0 g. Witte's pep- 

 tone and 5.0 g. K2HPO4 in 800.0 

 cc. distilled water, heating over the 

 steam for 20 minutes, and occa- 

 sionally stirring. 



(2) Filter thru a Schleicher and Schull 

 No. 588 folded filter. 



(3) Allow the filtrate to cool to 20°C. 

 and make up to 1000.0 cc. 



(4) Sterilize in the steamer on each of 

 3 successive days. 



(b) Clark and Lubs used the same 

 medium as in variant (a), but omitted 

 the K2HPO4 with or without the 

 addition of H3PO4 until the solution 

 is 0.075 molar NaOH. 



(c) Clark and Lubs used the same 

 medium as in variant (a) but used 5.0 

 to 8.0 g. glucose instead of 10.0 g. 



(d) Bunker, Tucker and Green gave the 

 same formula as variant (a) but used 

 5.0 g. glucose and 5.0 g. peptone and 

 omitted the phosphate. 



References: Lohnis (1913 p. 104), Rogers, 

 Clark and Evans (1914 p. 103), Clark and 

 Lubs (1915 pp. 162, 163), Bunker, Tucker 

 and Green (1918 p. 493), Committee 

 American Public Health Association 

 (1923 p. 107), (1925 p. 111). 



592. Mueller's Glucose Peptone Salt 

 Solution 

 Constituents: 



1. Water 2000.0 cc. 



2. Peptone 20.0 g. 



3. NaCl 10.0 g. 



4. MgS04 0.4 g. 



5. CaCla 0.2 g. 



6. K0HPO4 2.0 g. 



7. Glucose 2.0 g. 



8. Phenol red (0.02% soln.) ... 2.0 g. 

 Preparation : 



(1) Dissolve 2 in 1000.0 cc. of 1. 



(2) Dissolve 3, 4, 5, 6, 7 and 8 in 1000.0 cc. 

 of 1. 



(3) Mix (1) and (2). 



(4) Add NaOH until the medium is red 

 (pH = 7.8). 



Sterilization: Sterilize in autoclave at 10 



pounds for 10 minutes. 

 Use: To study the constituents essential 



for the growth of streptococci and pneu- 



mococci. The author reported this as a 



poor medium. 

 Reference: Mueller (1922 pp. 317, 325). 



593. Schukow's Basal Glucose Peptone Salt 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Dextrose 100.0 g. 



4. Potassium phosphate 5.0 g. 



5. MgS04 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 9.0 to 10.0% of one of the added 

 nutrients to (1). 



(3) Adjustment of reaction not specified. 



(4) Distribute into fermentation flasks 

 (sealed with H2SO4). 



Sterilization: Sterilize in a steamer. 



Use: To study the utilization of acids by 



yeast. After 85 days 0.124 g. of tartaric 



acid and 0.27 g. of malic acid were used. 



Acid determined by titration using 



litmus as an indicator. 

 Added nutrients: The author added 9.0 or 



10.0% of malic or tartaric acid. 

 Reference: Schukow (1896 p. 608). 



594. Henneberg's Glucose Peptone Salt 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KH,P04 (0.8%) 8.0 g. 



3. MgS04 (0.1%) 1.0 g. 



4. Glucose (5.0%) 50.0 g. 



5. Peptone (1.0 to 5.0%). 10.0 to 50.0 g. 

 Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



