170 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of lactic acid bacteria. 

 Reference: Henneberg (1903 p. 8). 



595. Harvey's Glucose Peptone Solution 



Constituents: 



1. Distilled water 960.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. Glucose 10.0 g. 



5. Litmus solution 40.0 cc. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Filter sterile (1) and adjust the 

 reaction if necessary. 



(3) Dissolve 10.0 g. glucose in 40.0 cc. of 

 litmus solution. 



(4) Mix (2) and (3). 



(5) Filter thru paper. 



(6) Steam 10 minutes. 



(7) Tube sterile (6) into sterile test tubes. 

 Sterilization: Sterilize (1) in the autoclave. 



Sterilize (6) by filtering thru a candle. 



Incubate to test sterility before use. 

 Use: General culture medium. 

 Reference: Harvey (1921-22 p. 109). 



596. Eijkman's Glucose Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose (10.0%) 100.0 g. 



3. Peptone (10.0%) 100.0 g. 



4. NaCl (5.0%) 50.0 g. 



Preparation: (1) Prepare a solution con- 

 taining 10.0% glucose, 10.0% peptone 

 and 5.0% NaCl. 



Sterilization: Not specified. 



Use: Water analysis and detection of 

 B. coli. The water under investigation is 

 placed into fermentation flasks and \ of 

 its volume of the medium is added. The 

 presence of uniform turbidity and gas 

 formation after 24 hours at 46 °C. indicates 

 the presence of B. coli. Indol may be 

 formed after 48 hours. 



Variants : 



(a) Truche and Cotoni enriched pneumo- 

 cocci in the following solution: 



1. Water 1000.0 cc. 



2. Peptone (Chapoteaut) 40.0 g. 



3. NaCl 5.0 g. 



4. Glucose 2.0 g. 



Chapoteaut's peptone cannot be 

 replaced by Witte's or Defresne's. 

 They specified that one-third part 



ascitic fluid might be added. Pneu- 

 mococci grew very well without the 

 ascitic fluid however. 



(b) Stewart and Stitt, determined the 

 Voges Proskauer reaction using 5.0 g. 

 NaCl, 20.0 g. glucose and 10.0 g. 

 Witte's peptone per liter. 



(c) Meyers studied H2S production using 

 30.0 g. of Witte's, Difco or Fairchild's 

 peptone, 5.0 g. glucose, 5.0 g. NaCl, 

 and 0.0 or 5.0 g. sucrose per liter. 



(d) Klimmer used 10.0 g. peptone, 5.0 g. 

 NaCl and 10.0 g. of glucose per liter. 



References: Eijkman (1904 p. 745), Truche 

 and Cotoni (1911 p. 480), Stewart (1917-18 

 p. 294), Myers (1920 p. 242), Stitt (1923 

 p. 36), Klimmer (1923 p. 221). 



597. Matzuschita's Glucose Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Meat peptone 



(Koch) 10.0 g. 



3. Gum arable 50.0 to 300.0 g. 



4. NaCl 5.0 g. 



5. Glucose 20.0 g. 



Preparation: 



(1) Dissolve 2 in 1. 



(2) Dissolve 3, 4 and 5 in (1). 



(3) Neutralize (indicator not specified). 

 Sterilization: Sterilize in the steamer on 



from 2 to 5 successive days for 15 to 30 

 minutes. Incubate for 2 days at 37°C. to 

 test sterility. 



Use: Cultivation of spore forming bacilli. 

 Clostridium butyricum, Bacillus oedem- 

 atis maligni, Bacillus anthracis sijmpto- 

 matici, Bacillus sporogenes, Bacillus 

 botulinus. 



Variants: The author used 1.0 to 3.0% gum 

 tragacanth instead of 50 to 300.0 g. gum 

 arable. 



Reference: Matzuschita (1902 p. 287). 



598. Gosio's Glucose Peptone Solution 



Constituents: 



1. Distilled water 3000.0 cc. 



2. Peptone 30.0 g. 



3. Dextrose 138.0 to 150.0 g. 



4. NaoCOs 1-6 g. 



5. CaC03 75.0 g. 



Preparation : 



(1) Dissolve 2 and 4 in part of 1. 



