CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



171 



(2) Dissolve 3 in the remainder of (1). 



(3) Mix sterile (1) and (2) when cool. 

 The addition of CaCOs not specified. 



Sterilization: Method not given. 



Use: Lactic acid production by vibrio. 



Variants: The author used the following 

 solution with 30.0 cc. normal soda solution 

 to study fermentation by Vibrio cholera 

 asiaticae. 



1. Water 3000.0 cc. 



2. Peptone (Witte) 30.0 g. 



3. Glucose 150.0 g. 



4. CaCO,, 75.0 g. 



Reference: Gosio (1894 p. 116, 2). 



599. Sears' Glucose Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 20.0 g. 



3. Glucose 5.0 g. 



4. NaCl 50 g. 



5. CaCOs 

 Preparation : 



(1) Dissolve 2 and 4 in 1. 



(2) Divide in two parts. 



(3) To one part of (2) add 1.0% (5.0 g.) 

 of pure glucose and a little CaCOs. 



(4) Distribute both portions in 200.0 cc. 

 lots into 300.0 cc. flasks. 



Sterilization: Sterilize in autoclave for 

 10 minutes at 15 pounds pressure. 



Use: To study nitrogen metabolism. Am- 

 monia determined by Folin's aeration 

 method, amino acid by Van Slyke's micro 

 method and total N by Kjeldahl-Gunning- 

 Arnold method. 



Reference: Sears (1916 p. 118). 



600. Ringer's Solution Broth (Park, 

 Williams and Krumwiede) 



Constituents : 



1. Water 



2. NaCl 



3. KCl 



4. CaCls 



5. Sodium bicarbonate. 



6. Glucose 



7. Peptone (1.0 or 



2.0%) 10.0 or 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Park, Williams and Krumwiede 



(1924 p. 122). 



601. Grimbert's Lactose Peptone Solution 

 (Robin) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Lactose (pure) 20.0 g. 



3. Peptone 5.0 g. 



4. Litmus 

 Preparation: 



(1) Boil 2 and 3 in 1, until solution is 

 complete. 



(2) Add lime water to neutralize. 



(3) Filter and readjust the reaction to 

 neutral to litmus if necessary. 



(4) The medium is tubed before steriliza- 

 tion if heated, but tubed in sterile 

 tubes if filtered to sterilize. 



(5) Add sufficient sterile litmus solution 

 to each tube to give color. 



Sterilization: Sterilize (3) by heating at 

 110° for 15 minutes or better by filtering 

 thru a clay candle. Sterilize the litmus 

 solution in the autoclave. 



Use: Differentiation of colon-typhoid 

 group. Colon bacilli gave an acid reac- 

 tion, typhoid bacilli did not change the 

 reaction. 



Reference: Robin (1901 p. 915). 



602. Levine's Lactose Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. Lactose (0.5%) 5.0 g. 



4. Brilliant green 

 Preparation: 



(1) Dissolve 2 and 3 in 1. 



(2) Add varying concentrations of bril- 

 liant green. 



Sterilization: Not specified. 



Use: Differentiation of Bact.coli and Bad. 

 aerogenes. Author reported that Bad. 

 aerogenes grew luxuriantly with 1-750,000 

 brilliant green. Bad. coU did not grow 

 at this dye concentration. If the peptone 

 content is decreased to 0.5% the anti- 

 septic action of brilliant green is very 

 markedly increased for both organisms. 



Variants: Used 0.5% peptone instead of 

 1.0%. 



Reference: Levine (1911 p. 22). 



603. Wagner and Monfort's Lactose Pep- 

 tone Solution 



Constituents: 



L Water 1000.0 cc. 



