172 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2. Peptone (2.0%) 20.0 g. 



3. Lactose (0.2%) 2.0 g. 



4. Gentian violet (0.001%). . . 0.01 g. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) The reaction is between pH 7.0 and 

 7.6. 



(3) Tube and plug with cotton. 

 Sterilization: Pasteurize at 70 to 80°C. for 



15 minutes. 



Use: Presumptive test for Bad. coli in 

 water analysis . Author reported that the 

 medium gave as good results as when the 

 usual 1.0 or 0.5% lactose was used. 0.001 

 to 0.00033% of gentian violet may be used. 



Variants: Used 0.001 to 0.00033% gentian 

 violet instead of 0.01%. 



Reference: Wagner and Monfort (1921 

 p. 207). 



604. Baginsky's Lactose Peptone Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Lactose 300.0 g. 



3. Peptone 

 Preparation : 



(1) Distribute distilled water in 25.0 cc. 

 quantities in small flasks that have 

 been sterilized at 140°C. 



(2) Add 5.0 g. of lactose and a small 

 amount (exact amount not given) of 

 peptone to each flask. 



Sterilization: Sterilize in flowing steam on 



3 successive days for ^ to 1 hour. 

 Use: To studj^ fermentation by B. lactis. 

 Reference: Baginsky (1888 pp. 434-462). 



605. Robin's Lactose Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Collas) 20.0 g. 



3. Sodium phosphate 0.2 g. 



4. NaCl 2.0 g. 



5. Soluble blue (1.0% soln.) ... 4.0 cc. 



6. Lactose 80.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 by boiling. 



(2) Add 4.0 cc. of 1.0% aqueous solution 

 of soluble blue ("blue soluble") to 

 boiling (1). 



(3) Add sufficient tenth normal KOH 

 solution to decolorize (2). 



(4) Add 80.0 g. of lactose to (3). 



(5) Filter. 



(6) Distribute in 10.0 cc. lots in Pasteur 

 flasks. 



Sterilization: Sterilize in the autoclave at 

 105° for 15 minutes. 



Use: Differentiation of colon and typhoid 

 groups. Author reported that coli strains 

 produced a blue colored medium, typhoid 

 cultures clouded the medium but gave no 

 change in color. 



Reference: Robin (1897 p. 49). 



606. Klein's Lactose Peptone Solution 



Constituents: 



1. Distilled water 



2. Peptone 



3. NaoHPOi 



4. NaCl 



5. Lactose 



6. Azolitmin 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Study of mutation in colon group. 

 Reference: Klein (1913 p. 100). 



607. Harvey's Lactose Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. Lactose (1.0%,) 10.0 g. 



5. Neutral red (sat. soln.) 



(1.0%) 10.0 cc. 



Preparation : 



(1) Dissolve 2 and 3 in 1 by boiling. 



(2) Dissolve 4 and 5 in (1) by heating. 



(3) Filter. 



(4) Distribute in test tubes containing 

 fermentation tubes. 



Sterilization: Sterilize on each of 3 suc- 

 cessive days at 100 °C. 



Use: General culture medium. Park, Wil- 

 liams and Krumwiede used the medium 

 in the e.xamination of water, sewage and 

 shellfish. 



References: Harvey (1921-22 p. 101), Park, 

 Williams and Krumwiede (1924 p. 132). 



608. Olszewski and Kohler's Lactose 

 Peptone Solution 



Constituents: 



1. Water 600.0 cc. 



2. Peptone 75.0 g. 



3. NaCl 50.0 g. 



