CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



173 



4. Lactose 75.0 g. 



5. Litmus solution 500.0 cc. 



Preparation : 



(1) Dissolve 75.0 g. peptone and 50.0 g. 

 NaCl in 600.0 cc. water. 



(2) Filter. 



(3) Dissolve 75.0 g. lactose in 500.0 cc. 

 litmus solution, made according to 

 Kubel and Tiemann with Kahlbaum's 

 litmus (method or reference not 

 given). 



(4) Boil (3) for 15 minutes. 



(5) Filter. 



(6) Add NaOH or soda solution to neu- 

 tralize if necessary. 



(7) Mix sterile (2) and (6) when ready 

 for use. 



(8) Tube. 



Sterilization: Method of sterilization of 

 (2), (6) or (8) not given. 



Use: Presumptive test for B. coli in water 

 analysis. Ten parts water under in- 

 vestigation were added to one part of 

 medium. B. coli turned the medium red. 



Reference: Olszewski and Kohler (1922 

 p. 305). 



609. Bacto Neutral Red Medium 

 (Dehydrated) 



Constituents: 



1. Distilled water 



2. Peptone, Bacto 30.0 g. 



3. Lactose, Bacto 6.0 g. 



4. KCl 8.0 g. 



5. Neutral red 0.08 g. 



Preparation : 



(1) Dissolve 44.0 g. of Bacto neutral red 

 medium in 1000.0 cc. of distilled water. 



(2) If sterilized 20 minutes at 15 pounds 

 pressure pH = 6.7. 



Sterilization: Sterilize in the usual manner. 

 Reference: Digestive Ferments Co. (1925 

 p. 13). 



610. Fischer and Andersen's Lactose 

 Peptone Solution 



Constituents: 



1. Water (96.5%) 965.0 cc. 



2. Peptone (Witte) (1.0%) 10.0 g. 



3. Lactose (2.0%) 20.0 g. 



4. NaCl (0.5%) 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjustment of reaction not given. 



(3) Distribute in 50.0 cc. flasks. 



Sterilization: Not specified. 



Use: To study acid production by Bac- 

 terium coli. 



Reference: Fischer and Andersen (1912 

 p. 289). 



611. Baginsky's Lactose Peptone Salt 

 Solution 



Constituents: 



1 . Distilled water 750.0 cc. 



2. Lactose 36.0 g. 



3. Peptqne (siccum) 8.0 g. 



4. K2HPO4 1-6 g. 



5. CaCl, 0.15 g. 



6. MgS04 0.3 g. 



7. CaCOs 0.02 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute into sterile Erlenmeyer 

 flasks. 



Sterilization: Sterilize in flowing steam for 



4 days for | to 1 hour. 

 Use: To study fermentation by B. lactis. 

 Reference: Baginsky (1888 pp. 434-462). 



612. Bendick's Phenolphthalein Sucrose 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. NaaCOa 1-0 g. 



5. Sucrose 5.0 g. 



6. Phenolphthalein (Sat. 50.0% 



ale. soln.) 5.0 cc. 



Preparation: 



(1) Dissolve 2 and 3 in 1 by heating over 

 a free fl^-me. 



(2) Add NazCOs to neutralize to phenol- 

 phthalein. 



(3) Add 1.0 g. of NajCOs to (2), and boil 

 over a free flame. 



(4) Filter thru a double layer of filter 

 paper. 



(5) Add 5.0 g. sucrose and 5.0 cc. of a 

 50.0% saturated alcoholic solution of 

 phenolphthalein to (4). 



(6) Medium should be moderately deep 

 red. 



Sterilization: Sterilize in the Arnold for 

 20 minutes if the medium is to be used at 

 once. If not to be used at once, sterilize 

 in the Arnold for 15 minutes on each of 3 

 successive days. 



