CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



175 



3. KH2PO4 100.0 g. 



4. MgS04 0.2 g. 



5. Calcium phosphate 0.2 g. 



6. Sucrose 150.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Flask. 



Sterilization: Sterilize in an autoclave on 

 successive days with temperatures of 

 115° to 120°C. (15 to 18 pounds pressure). 



Use: Cultivation of yeast {Saccharomyces 

 cereviseae). Author reported a more 

 rapid growth than in beer wort. 



Reference: Alutchler (1905-06 p. 34). 



617. Waksman's Sucrose Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone 20.0 g. 



3. K2HPO4 1.0 g. 



4. KCl 0.5 g. 



5. MgS04 0.5 g. 



6. FeS04 0.01 g. 



7. Cane sugar 30.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute (1) in 100.0 cc. portions 

 in 200.0 cc. Erlenmeyer flasks. 



Sterilization: Sterilize at 15 pounds for 



15 minutes. 

 Use: Cultivation of soil actinomyces and 



fungi. 

 Variants: Author used 1.0, 3.0, 5.0 or 20.0% 



sucrose. 

 Reference: Waksman (1918 p. 510). 



618. Zikes' Sucrose Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Sucrose 100.0 g. 



3. K2HPO4 1.0 g. 



4. MgS04 0.3 g. 



5. Peptone 2.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) pH = 6.0603, CH = 8.7 X 10-^ 

 Sterilization: Not specified. 



Use: Volutin formation by Saccharomyces 

 Frohberg, wine yeasts, Saccharomyces 

 anamensis, Saccharomyces ilicis Saccharo- 

 myces Will (Bajanus), Pichia membranae- 

 faciens, Cholera m,ycoderma, Manilla 

 Candida, Oidium lactic, Endomyces 

 Magnusi. 



Reference: Zikes (1922 p. 29). 



619. Behrens' Basal Sucrose Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Cane sugar 100.0 g. 



3. Peptone 5.0 g. 



4. MgS04 1.0 g. 



5. K2HPO4 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Distribute in 100.0 cc. lots. 



(3) Add one of the added nutrients as 

 indicated below. 



Sterilization: Method not given. 



Use : To study the effect of organic acids on 

 growth of Penicillium glaucum, Pencil- 

 Hum luteum and Botrytis spores. 



Added nutrients: The author added one of 

 the following to each lot: 

 malic acid 0.5, 1.0, 1.5, 2.0 or 5.0 g. 



phloroglucine 0.5 or 1.0 g. 



hydrochinon 0.5 or 1.0 g. 



tartaric acid 7.35 g. 



citric acid 6.86 g. 



Reference: Behrens (1898 pp. 644, 739). 



620. Bokorny's Sucrose Somatose Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Somatose 2.5 g. 



3. Sucrose 50.0 g. 



4. KH2PO4 2.0 g. 



5. MgS04 10 g- 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of yeast. 

 Reference: Bokorny (1917 p. 364). 



621. Trommsdorff's Sucrose Peptone 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. MgS04 0.05 g. 



3. CaC04 0.05 g. 



4. K2HPO4 0.1 g. 



5. NaCi 0.1 g. 



6. FeCl3 0.001 g. 



7. Peptone (0.1%) (1.0 g. or 



0.5%) 5.0 g. 



8. Sucrose (0.5%) 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 



7 and 8 in 1. 

 Sterilization: Not specified. 



