176 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of Leptomitus. Growth 

 very good on this medium. Better 

 growth using 0.5% peptone than 0.1%. 



Reference: Trommsdorff (1918 p. 65). 



622. Vierling's Sucrose Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. CaClo 0.1 g. 



4. MgS04 0.1 g. 



5. FeCls trace 



6. NaCl trace 



7. Peptone 10.0 g. 



8. Sucrose 10.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 and 



8 in 1. 



Sterilization: Not specified. 



Use: To determine invertase production by 

 mycobacteria. Test for reducing sugars 

 with Fehling's solution. Very small 

 amounts of sugar if any were reduced. 



Reference: Vierling (1920 p. 206). 



623. Baginsky's Starch Peptone Solution 



624. Dumas' Glycogen Peptone Solution 



Constituents: 



1. Distilled water 100.0 cc. 



2. Peptone (Chapoteaut) 1.0 g. 



3. NaCl 0.5 g. 



4. Glycogen .5 g. 



5. Litmus 

 Preparation : 



(1) Dissolve 2 in 1 by heating. 



(2) Dissolve 3 in (1). 



(3) Neutralize to litmus. 



(4) Add 5.0 cc. of normal soda per liter 

 (0.5 cc. for 100.0 cc). 



(5) Boil slowly for several minutes. 



(6) Filter thru wet paper. 



(7) Add 4 to (6) and shake a few minutes. 



(8) Add drop by drop enough litmus 

 solution to give the desired color. 



Sterilization: Sterilize for 15 minutes at 

 110°C. 



Use : To study the fermentation of glycogen 

 by the cholera vibrio. Dumas reported 

 that glycogen was fermented, the medium 

 was turned red, but gas was not formed 

 at the end of 18 or 24 hours. 



Reference: Dumas (1919 p. 547). 



625. Kodama and Takeda's Starch Peptone 



Solution 



Constituents: 



1. Water ■ 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. Potato starch 5.0 g. 



4. 10.0% soda solution 20.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Distribute into sterile tubes. 

 Sterilization: Sterilize on 2 or 3 successive 



days (method not given). 



Use: To determine digestion of starch. 

 The author added 5.0 cc. of Lugol's 

 solution (1.0 g. iodine 2.0 g. KI in 

 100.0 cc.) water to the cultures. After 

 24 hours the starch had nearly dis- 

 appeared from the cholera cultures and 

 only a yellow color appeared when the 

 iodine solution was added. The cholera- 

 like cultures used less starch and the 

 remaining cultures used no starch at all 

 as was indicated by the amount of starch 

 left in the bottom of the tube and the 

 color change when iodine solution was 

 added. 



Reference: Kodama and Takeda (1922 

 p. 514). 



626. Khouvine's Cellulose Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. NaCl 1.0 g. 



4. Peptone (Pancreatic) 1.0 g. 



5. Cellulose 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Reaction is neutral. 



(3) Distribute in 5.0 cc. lots in tubes. 



