CULTURE MEDIA FOR CULTIVATION OF MICRO ORG AXISMS 



177 



(4) Add 1 square centimeter of cellulose 

 in the form of Berzelius filter paper 

 or a little cellulose precipitated after 

 having been dissolved in Schweitzer's 

 reagent to each tube. 

 Sterilization: Sterilize for 15 minutes at 



110°C. 

 Use: Isolation and enrichment of B. cellu- 

 lose dissolvens 

 Variants: The author gives the following 

 variants : 



(a) Addition of 2.0 g. glucose. 



(b) Used 0.5 g. K2HPO4 instead of 1.0 g., 

 added 0.5 g. Na2HP04, and 2.0 g. of 

 CaCOs. 



(c) Same as (b) with 2.0 g. of glucose. 



(d) Used traces of NaCl instead of 1.0 g., 

 added 0.5 g. MgS04, traces of CaCOj 

 and some gum arable (amount not 

 given). 



(e) Same as (d) with 2.0 g. glucose. 

 Reference: Khouvine (1923 p. 713). 



627. Vierling's Cellulose Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (3.0%) 30.0 g. 



3. CaCOa (0.1%) 1.0 g. 



4. Filter paper 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Place strips of filter paper in flasks. 



(3) Pour (1) into (2). 



(4) Seal the flasks with paraffin after 

 inoculation. 



Sterilization: Not specified. 



Use: Decomposition of cellulose by myco- 

 bacteria. Filter paper showed no signs 

 of being attacked. Growth occurred, 

 however. 



Variants: The author gave the following 

 variants: 



(a) Used 2.0% peptone and added 1.0% 

 glucose. 



(b) Used 2.0% peptone and added 0.4% 

 KNO3. 



Reference: Yierling (1920 p. 206). 



628. Matzuschita's Pyrogallic Acid Bouillon 



Constituents: 



1. Water 1000.0 cc. 



2. Meat peptone (Koch) 10.0 g. 



3. NaCl 5.0 g. 



4. Dextrose (2.0%) 20.0 g. 



5. Glycerin (5.0%) 50.0 g. 



6. "Eikonogen" (0.1%) 1.0 g. 



7. Hydrochinone (0.1%) 1.0 g. 



8. Pyrogallic acid (0.1%) 1.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Neutralize. Indicator not specified. 

 Sterilization: Sterilize in the steamer on 



from 2 to 5 successive days for 15 to 30 

 minutes. Incubate for 2 days at 37^0. 

 to test sterility. 



Use: Cultivation of spore forming bacilli. 

 Clostridium butyricum, Bicillus oedem- 

 atis maligni, Bicillus anthracis sympto- 

 matica, Bacillus sporogenes, Bacillus 

 botulinus. 



Reference: Matzuschita (1902 p. 287). 



629. Bezanfon's Glycerol Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose 23.0 g. 



3. Glycerol 20.0 g. 



4. Peptone 10.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Hyphomycetes, Sporo- 



trichum heurmanni . 

 Reference: Bezangon (1920 p. 644). 



630. Behrens' Tartrate Peptone Solution. 



Constituents: 



1. Water 1000.0 cc. 



2. Starch 10.0 g. 



3. Cane sugar 20.0 g. 



4. Peptone 5.0 g. 



5. Tartaric acid 5.0 g. 



6. K2HPO4 2.0 g. 



7. MgS04 1.0 g. 



8. Copper sulphate 0.0, 0.1, 0.02, 0.1, 

 0.2, 0.5 or 1.0 g. (per 100.0 cc.) 



Preparation : 



(1) DLssolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute in 100.0 cc. lots. 



(3) Prepare a 10.0% solution of copper 

 vitrol. 



(4) AddO.O, 0.1, 0.2, 1.0, 2.0, 5.0 or 10.0 cc. 

 of (3) to each flask. 



Sterilization: Method not given. 



Use : To show influence of copper on growth 

 of Oidium fructigenum. Growth is in- 

 hibited by a dilution of about 1.0 g. of 

 copper sulphate per 109.0 cc. (10.0 cc. of a 

 10.0% solution). 



