178 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Variants: Author used 50.0 g. sucrose in- 

 stead of 20.0 g. and omitted the starch. 

 Reference: Behrens (1898 pp. 775-776). 



631, 



Dombrowski's Glucose Peptone 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. Glucose 50.0 g. 



4. K2HPO4 2.0 g. 



5. MgS04 10 g. 



6. NaCl 1.0 g. 



7. Lactic acid 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Acidify slight!}^ with lactic acid. 

 Sterilization: Not specified. 



Use: Cultivation of milk yeasts. 

 Reference: Dombrowski (1910 p. 358). 



632. Zumstein's Citric Acid Peptone 

 Solution (Killer) 



Constituents: 



1. Water 



2. Peptone 



3. Glucose 



4. Citric acid 



5. MgS04-7H20 



6. KH2PO4 



1000.0 cc. 



5.0 g. 



5.0 g. 



2.0 g. 



0.2 g. 



0.5 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



inl. 

 Sterilization: Not specified. 

 Use: Cultivation of flagellates, organisms 



from the soil, etc. 

 Variants : Dilute the medium 5 to 10 times 



for cultivation of protozoa. 

 Reference: Killer (1913 p. 522). 



633. Seitz's Double Sugar Peptone Solution 

 (Harvey) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Lactose 20.0 g. 



3. Glucose 0.4 g. 



4. Na2HP04 0.5 g. 



5. Ammonium sulphate 1.0 g. 



6. Sodium citrate 2.0 g. 



7. NaCl 5.0 g. 



8. Peptone 0.05 g. 



9. Azolitmin 0.05 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 



8 and 9 in 1. (Method not given.) 

 Sterilization: Not specified. 



Use: Substitute for litmus milk. 



Variants: Klimmer specified the use of 

 Witte's peptone and used 0.25 g. Kahl- 

 baum's azolitmin or added 1 or 2 drops of a 

 saturated watery solution of China blue. 

 He used the medium as a whey substitute. 



References: Harvey (1921-22 p. 109), Klim- 

 mer (1923 p. 208). 



634. Miiller, Thurgau and Osterwalder's 

 Malic Acid Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. H2KPO4 10 g. 



4. MgS04 0.2 g. 



5. CaCl2 0.2 g. 



6. Malic acid 1.0 g. 



7. Levulose 20.0 g. 



Preparation : 



(1) Boil 10.0 liters of tap water. (Given 

 as ten liters and not one.) 



(2) Filter. 



(3) Dissolve 2, 3, 4, 5, 6 and 7 in (2). 



(4) Tube. 



Sterilization: Sterilize the fractional 

 method. 



Use: Cultivation of Bacterium mannito- 

 poeum and bacteria from wine and fruit 

 wine. Authors reported that when po- 

 tassium malate is present a larger ratio 

 of the acid produced is lactic (non volatile) 

 rather than acetic. 



Variants: Authors added 1.0% potassium 

 malate . 



Reference: Miiller, Thurgau and Oster- 

 walder (1912-13 p. 161). 



635. Ayers, Rupp and Johnson's Glucose 

 Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Pepsin (Park Davis & Co.).. 5.0 g 



3. Peptone (Park Davis & Co.) 5.0 g 



4. K2HPO4 3.0 g 



5. NaCl 5.0 g 



6. Glucose 2.5 g 



7. Soap (castor oil) 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjust to pH = 7.6. 



(3) Add sufficient quantity of castor oil 

 soap (sodium ricinolate) to lower the 

 surface tension 50 and 43 dynes. 



