CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



179 



Sterilization: Method not specified. 



Use: To study effect of surface tension on 

 growth of streptococci. Author reported 

 that in general when the surface tension 

 was reduced to from 40 to 41 dynes the 

 growth of streptococci was prevented. 



Reference: Ayers, Rupp and Johnson (1923 

 p. 204). 



636. Marmier's Glycerol Protease Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 40.0 g. 



3. NaCl 15.0 g. 



4. Sodium phosphate 0.5 g. 



5. Potassium phosphate 0.2 g. 



6. Glycerol 40.0 g. 



Preparation : 



(1) Dissolve the peptone in a certain 

 amount of water. 



(2) Add enough (NH4)2S04 to (1) so that 

 the solution is saturated at 100°C. 



(3) Boil and then filter. 



(4) Dialyse in a current of water for 

 40 hours. 



(5) The liquid may then be reduced to 

 3.0% peptone and sterilized at 115° 

 if not ready for use. 



(6) Instead of dialysing the liquid free 

 from (NH4)2S04 as in step (4), 

 barium hydrate may be added in such 

 an amount so that all the sulphate be 

 precipitated. Filter and keep the 

 filtrate at a temperature near boiling 

 and pass a current of air thru it to 

 free it of ammonia. When free of 

 ammonia pass a current of CO2 

 thru the filtrate to remove the excess 

 of barium. Filter. 



(7) Make up the ammonia free filtrate (5) 

 or (6) up to 1 liter and dissolve 3, 4, 5 

 and 6 in it. 



(8) Filter and distribute the filtrate in 

 250.0 cc. Pasteur flasks. 



Sterilization: Sterilize at 110°C. (time not 



specified). 

 Use: Anthrax toxin production. Marmier 



reported this medium as being non-toxic 



for animals. 

 Reference: Marmier (1895 p. 536). 



637. Franklin and Fox's Glycerol Peptone 

 Solution (Owen) 



Constituents: 

 1. Water 5000.0 cc. 



2. Glycerol 60.0 g. 



3. Peptone 2.0 g. 



4. Calcium carbonate (pre- 

 cipitated) 30.0 g. 



5. Potassium phosphate 0.5 g. 



6. MgS04 1.0 g. 



7. CaCls 0.5 g. 



Preparation : 



(1) Dissolve 5, 6 and 7 in 1. 



(2) Dissolve 2, 3 and 4 in 2000.0 cc. of (1). 



(3) Divide into liter portions. 

 Sterilization: Sterilize using the inter- 

 mittent method. 



Use: To determine amount of alcohol 

 formed in the fermentation of glycerin 

 by Bacillus saccharalis. Author reported 

 that from a liter culture 50.0 cc. of a 

 1.707% alcohol solution could be obtained. 



Reference: Owen (1916 pp. 239-241). 



638. Harvey's Glycerol Peptone Solution 



Constituents: 



1. Peptone (2.0% soln.) 870.0 cc. 



2. Glycerol 100.0 cc. 



3. NaOH (normal soln.) 30.0 cc. 



Preparation: 



(1) Prepare a 2.0% peptone solution. 



(2) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of V. cholerae. 

 Reference: Harvey (1921-22 p. 87). 



639. Waksman's Basal Glycerol Peptone 



Solution 



Constituents: 



1. Water 



2. Glycerol 



3. K2HPO4 



4. KCl 



5. MgS04 



6. FeS04 



7. Peptone (Witte's) 



Preparation: 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 2.0 g. of 

 one of the added nutrients in 1. 



(2) Tube in 10.0 to 12.0 cc. quantities. 

 Sterilization: Sterilize at 15 pounds pres- 

 sure for 15 minutes. 



Use: To study metabolism of actinomy- 



cetes. 

 Added nutrients: The author added 2.0 g. 



of one of the following salts: 



NaNOa 



NaNOz 



