180 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(NH4)2S04 



(NH4)2C03 



Reference: Waksman (1920 p. 3). 



640. Capaldi and Proskauer's Mannitol 

 Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Mannitol (0.1%) 1.0 g. 



3. Peptone (Witte) (0.5 



or 2.0%) 5.0 or 20.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 



Sterilization: Not specified. 



Use: To study the fermentation of man- 

 nitol by the colon-typhoid group. 



Variants: The author gave the following 

 variants : 



(a) Substituted Carne, Aschman, Chapo- 

 teaut, Driisen, Hemialbumose, soma- 

 tose or glutin peptone (Paal) for 

 Witte's peptone. 



(b) Used 2.0% Witte's peptone and added 

 sufficient litmus to give a color. 

 Mannitol was fermented with strong 

 acid reaction by the typhoid bacilli 

 after 20 hours. The following salts 

 were added in 0.1% strength. Their 

 presence did not inhibit development 

 of the organisms to any extent: 



aluminum acetate 



potassium antimonate 



sodium arsenate 



lead acetate 



borax 



cadmium chloride 



chrome alum 



iron alum 



potassium ferrocyanate 



copper acetate 



manganese sulphate 



ammonium molybdate 



nickel sulphate 



sodium nitroprusside 



palladium chloride 



sublimate 



silver sulphate 



strontium nitrate 



thallium 



uranium nitrate 



bismuth citrate 



zinc sulphate 



zinc chloride 

 (c) Harvey added 47.5 cc. litmus solution 

 to a solution containing 2.0% pep- 

 tone and 0.1% mannitol. 



References: Capaldi and Proskauer (1894 

 p. 464), (1896 p. 472), Harvey (1921-22 

 p. 111). 



641. Omeliansky's Formate Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Calcium formate 20.0 g. 



3. Peptone 2.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Flask. 



Sterilization: Method not given. 



Use : To study decomposition of formic acid 

 by Bacterium formicicum. About 3 

 times more H2 was formed than CO2. 

 Inoculate and seal with a rubber stopper 

 fitted with a tube containing a mercury 

 seal. About 250.0 cc. of air was sealed 

 into the flask with the culture. 



Reference: Omeliansky (1903-04 p. 188). 



642. Voges and Proskauer's Basal Citrate 

 Peptone Solution 



Constituents: 



1. Distilled water 100.0 cc. 



2. Na2HP04 0.37 g. 



3. KH2PO4 0.14 g. 



4. CaCl2 0.04 g. 



5. KCl 0.3 g. 



6. Magnesium citrate 0.01 g. 



7. Peptone (1.0%) 1.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 1.0% of 

 one of the added nutrients. 



(2) Neutralize with soda. 

 Sterilization: Not given. 



Use: Study fermentation of hemorrhagic 



septicemia bacteria. Growth reported 



as being more luxuriant than in bouillon. 



Added nutrients: The authors added 1.0% 



of one of the following: 



glucose dextrin 



mannose starch (potato) 



levulose glycerol 



sucrose adonitol 



lactose dulcitol 



maltose mannitol 



raffinose 

 Variants: The authors used the basic solu- 

 tion without any additions. 

 Reference: Voges and Proskauer 

 pp. 22, 28). 



