CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



181 



643. Behren's Basal Citrate Acid Peptone 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 2.0 g. 



3. MgS04 1.0 g. 



4. Citric acid 10 g. 



5. Peptone 20.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Distribute in 200.0 cc. lots in flasks. 



(3) Add one of the combinations given 

 under added nutrients to each flask. 



Sterilization: Method not given. 

 Use: Cultivation of botr3^tis spores. 



Added nutrients: The author added one of 



the following to 200.0 cc. of the basic 



solution: 



sucrose 0.5 g. 



salicin 4.0 g. 



salicin 1 g. 



+ glycerol 1 .0 g. 



glycerol 1 -0 g. 



Reference: Behrens (1898 p. 578). 



644. V. Freudenreich and Jensen's Lactate 



Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) 20.0 g. 



3. K2HPO4 2.0 g. 



4. NaCl 5.0 g. 



5. Calcium lactate 20.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Enrichment of propionic acid formers 



from cheese. Medium is inoculated with 



a cheese emulsion. 

 References: V. Freudenreich and Jensen 



(1907 p. 529), Boekhout and Ott de Vries 



(1918 p. 130). 



645. Baginsky's Lactate Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Sodium lactate 10.0 g 



3. Peptone 2.5 g 



4. K2HPO4 0.1 g 



5. CaClj 0.02 g 



6. MgS04 0.02 g 



7. CaC03 0.02 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Distribute in sterile flasks. 

 Sterilization: Sterilize in flowing steam on 



3 consecutive days for 5 to 1 hour. 

 Use: To study fermentation by B. lactis. 

 Reference: Baginsky (1888 pp. 434-462). 



646. Harvey's Tartrate Peptone Solu-^ion 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. K2HPO4 3.0 g. 



4. Peptone 30.0 g. 



5. Ferric tartrate (2.0% soln.). 10.0 cc. 

 Preparation: 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Tube in 10.0 cc. quantities. 



(3) Add 0.1 cc. of a 2.0% neutral ferric 

 tartrate solution. A yellowish pre- 

 cipitate forms. 



Sterilization: Method not given. 

 Use: General culture medium. 

 Reference: Harvey (1921-22 p. 107). 



647. Harrison and Vanderleck's Aesculin 

 Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte) 23.0 g. 



3. Aesculin (Merck) 0.5 g. 



4. Iron citrate scales (Merck). 0.5 g. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjust the reaction to +0.6. 



(3) Tube in 10.0 cc. lots. 

 Sterilization: Not specified. 



Use: Detection of B. coli and B. typhosus. 

 B. coli gives blackened medium. Me- 

 dium without bile salts blackened quicker 

 than with the bile salt. 



Variants: The authors give the following 

 variants: 



(a) Addition of 3.0 g. sodium tauro- 

 cholate. 



(b) Use of 10.0 g. peptone instead of 

 20.0 g. Witte's peptone, 2.5 g. iron 

 citrate scales (Merck) instead of 

 0.5 g., and 1.0 g. of aesculin instead 

 of 0.5 g. 



(c) Same as (b) but add 2.5 g. bile salt 

 (sodium taurocholate). 



Reference: Harrison and Vanderleck (1909 

 pp. 616, 622). 



