182 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



SUBGROUP I-C. SECTION 10 



Liquid media or basal solutions contain- 

 ing peptone or similar commercial digests 

 and additional nitrogen compounds of 

 known chemical composition; organic car- 

 bon compounds added. 

 Ai. Organic nitrogen present only as 



peptone. 

 Bi. Inorganic nitrogen supplied as ammo- 

 nium salt. 

 Ci. Ammonium chloride added. 



Jones' Glucose Peptone Solution. . . . 648 

 Lustig's Ammonium Chloride Pep- 

 tone Solution 649 



Cathelineau's Basal Ammonium Sul- 

 phate Peptone Solution 650 



C2. Ammonium sulphate added. 



Grimbert's Basal Peptone Salt Solu- 

 tion 651 



Beijerinck's Malate Peptone Solu- 

 tion 652 



Klecki's Lactose Peptone Solution. . . 653 

 Seitz's Lactose Peptone Solution. . . . 654 

 C3. Ammonium salts of phosphoric acid 

 added. 



Hauman's Pectin Peptone Solution. . 655 

 Charpentier's Sucrose Peptone Solu- 

 tion 656 



B2. Inorganic nitrogen supplied as nitrate. 

 Ci. Additional organic carbon supplied as 



carbohydrates. 

 Di. Monosaccharides added. 

 Lustig's Nitrate Peptone Solution.. 657 

 Stoklasa's Nitrate Peptone Solution. 658 

 Stutzer's Nitrate Glucose Solution. . 659 

 Duchacek's Nitrate Peptone Solu- 

 tion 660 



D2. Polysaccharides added. 

 Khouvine's Cellulose Nitrate Solu- 

 tion 661 



Omeliansky's Cellulose Peptone Solu- 

 tion (Khouvine) 662 



C2. Additional Organic carbon supplied as 

 alcohols or organic acids. 

 Maassen's Nitrate Peptone Solution. 663 

 Wassermann's Nitrate Peptone Solu- 

 tion 664 



Duchacek's Nitrate Tartaric Acid 



Solution 665 



A2. Organic nitrogen present in addition to 



peptone. 

 Bi. Amino acids added. 

 Ci. Amino nitrogen supplied as asparagin. 



Hiss' Basal Asparagin Peptone Solu- 

 tion 666 



Henneberg's Basal Asparagin Pep- 

 tone Solution 667 



Nicole and Refik-Bey's Asparagin 

 Peptone Solution 668 



Thoinnessen's Asparagin Peptone 

 Solution 669 



Bokorny's Asparagin Peptone Solu- 

 tion 670 



Reichenbach's Lactose Peptone 

 Solution (Quantz) 671 



Frankel's Asparagin Peptone Solu- 

 tion (Tanner) 672 



C2. Amino nitrogen supplied as tyrosine. 



Berthelot's Tyrosine Peptone Solu- 

 tion 673 



C3. Amino nitrogen supplied as trypto- 

 phane. 



Tryptophane Broth (A. P. H. A.). . . . 674 



Frieber's Tryptophane Peptone Solu- 

 tion 675 



Harvey's Tryptophane Peptone Solu- 

 tion 676 



B2. Organic nitrogen other than amino 



acids added. 

 Ci. Containing urea. 



Hiss' Basal Urea Peptone Solution.. 677 



Killer's Urea Peptone Solution 678 



Cunningham's Urea Peptone Solu- 

 tion 679 



C2. Containing hippuric acid. 



Ayers and Rupp's Hippurate Peptone 

 Solution 680 



Ayers and Rupp's Pepsin Peptone 



Solution 681 



C3. Containing Bile Salts. 



MacConkey's Bile Salt Peptone Solu- 

 tion 682 



Harrison and van der Leek's Bile Salt 

 Peptone Solution 683 



648. Jones' Glucose Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NH4CI 5.0 g. 



3. Glucose 5.0 g. 



4. Peptone 10.0 g. 



5. Calcium lactate 10.0, 20.0, 30.0, 40.0, 

 50.0, 60.0, 70.0, 80.0, 90.0, or 100.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Method not given. 

 Use: To study fermentation by B. proteus. 

 The author reported the more lactate 



