CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



183 



salt present, the less acid and gas were 

 formed. 



Variants: The author used sodium or am- 

 monium lactate instead of calcium 

 lactate. 



Reference: Jones (1916 p. 40). 



649. Lustig's Ammonium Chloride Peptone 

 Solution 



Constituents: 



1. Water 4000.0 cc. 



2. Potassium phosphate 1-0 g. 



3. MgS04 (crystals) 0.2 g. 



4. CaClj (fusum) 0.1 g. 



5. Invert sugar 5.0 g. 



6. NH4CI 5.0 g. 



7. Peptone 10 g. 



8. CaCOs 15.0 g. 



Preparation : 



(1) Prepare a salt solution by dissolving 

 1.0 g. potassium phosphate, 0.2 g. 

 MgS04 (crystals) and 0.1 g. CaCh 

 (fusum), in 1000.0 cc. water. 



(2) Prepare an invert sugar solution by 

 dissolving 5.0 g. cane sugar in 1000.0 

 cc. water and inverting. 



(3) Prepare an ammonium chloride solu- 

 tion by dissolving 5.0 g. NH4CI in 

 500.0 cc. water. 



(4) Mix 400.0 cc. (1), 240.0 cc. of (2) and 

 200.0 cc. of (3). 



(5) Add and dissolve in (4), 1.0 g. pep- 

 tone and 15.0 g. of CaCOs (puriss). 



(6) Dilute (5) to 4000.0 cc. with water. 



(7) Distribute into well sterilized 100.0 

 cc. flasks in 30 to 50.0 cc. quantities. 



Sterilization: Sterilize well on each of two 



successive days for an hour. 

 Use: To study the oxidation of ammonia 



by a red pigment producing organism 



isolated from water, and by bacteria from 



the soil. 

 References: Lustig (1890 p. 38), Frankland 



and Frankland (1889 p. 376). 



650. Cathelineau's Basal Ammonium 

 Sulphate Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Sodium phosphate 



3. Sodium potassium tartrate 



4. (NH4)2S04 5.0 g. 



5. Peptone 10.0 g. 



6. CaCOa 



Preparation : 



(1) Dissolve 5.0 g. of a mixture of sodium 

 phosphate and sodium potassium 

 tartrate (Seignette's salt) in 1. 



(2) Dissolve 5.0 g. (NH4)iS04, 10.0 g. of 

 peptone and one of the added nu- 

 trients in (1). 



(3) Add an excess of CaCOa. 

 Sterilization: Not specified. 



Use: Cultivation of Bacillus viridis 

 (Lesage). 



Added nutrients : The author added 2.0% of 

 one of the following materials: 



glucose succinic acid 



levulose tartaric acid 



lactose lactic acid 



galactose citric acid 



maltose butyric acid 



sucrose glycocolic acid 



mannitol malic acid 



glycerol aspartic acid 



Reference: Cathelineau (1896 p. 229). 



651. Grimbert's Basal Peptone Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Ammonium phosphate 0.4 g. 



3. MgS04 0.4 g. 



4. Potassium phosphate 0.2 g. 



5. (NH4)2S04 0.2 g. 



6. KNO3 0.2 g. 



7. Peptone (dry) 2.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 3.0 to 5.0% 

 of one of the added nutrients in 1. 



(2) Tube or flask. 



Sterilization: Sterilize at 120°C. for 15 to 



45 minutes depending on the size of the 



flask. 

 Use: To study the fermentation ability of 



Bacillus orthobutylicus. 

 Added nutrients: The author added 3.0 to 



5.0% of any fermentable material. 

 Reference: Grimbert (1893 p. 359). 



652. Beijerinck's Malate Peptone Solution 



Constituents: 



1. Water (ditch) 1000.0 cc. 



2. Potassium malate 2.5 g. 



3. Peptone (siccum) 2.5 g. 



4. Mohr salt 1.0 g. 



(FeS04(NH4)2S04-6H20) 



