184 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2, 3, and 4 in ditch water 

 ("Grabenwasser"). 



(2) Make alkaline with Na2C03. 

 Sterilization: Not specified. 



Use: To study reduction of sulphates by 



Spirillum tenue. 

 Reference: Beijerinck (1895 p. 107). 



653. Klecki's Lactose Peptone Solution 



Constituents : 



1. Water 8000 to 10,000.0 cc. 



2. Calcium lactate 225.0 g. 



3. Ammonium phosphate. . . 0.75 g. 



4. Potassium phosphate 0.4 g. 



5. MgS04 0-4 g- 



6. (NH4)2S04 0-2 g- 



7. Peptone 200.0 g. 



8. Lactose 500.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Add some chalk to each flask. 

 Sterilization: Method not given. 



Use: To study fermentations in cheese. 

 The medium was inoculated with a piece 

 of "Quargel" cheese. Vibrio butyrique, 

 Bacillus saccharobutyricus, and other 

 organisms developed. Medium was used 

 under anaerobic as well as aerobic 

 conditions. 



Reference: Klecki (1896 p. 173). 



654. Seitz's Lactose Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Lactose 20.0 g. 



3. Glucose 0.4 g. 



4. NaoHP04" 1-0 g. 



5. Sodium citrate (tribasic) . . 2.0 g. 



6. NaCl 5.0 g. 



7. Peptone (Witte sice) 0.05 g. 



8. Azolitmin (Kahlbaum's) . . 0.25 g. 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 



(2) The reaction should be neutral. 



(3) The medium is bluish violet in color. 

 Sterilization: Do not sterilize longer than 



30 minutes at 100 °C. 

 Use : Differential medium for colon-typhoid 

 group. Author reported that the colon 

 group produced acid after 5 hours. 

 Typhoid bacilli produced a little acid 

 after 24 hours by dextrose decomposition. 

 Dysentery and paratyphoid organisms 



acted as typhoid. Bacillus faecalis alcali- 

 genes produced an alkaline reaction. 

 Reference: Seitz (1912 p. 434). 



655. Hauman's Pectin Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Pectin 10.0 g. 



3. Peptone 10 g. 



4. Ammonium phosphate 10 g. 



5. K0SO4 0-5 



6. MgS04 0-2 _ 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of B. coli, B. fluorescens, 



Cladosporium herbarum, Aspergillus 



niger, Penicillium glaucum. 

 Reference: Hauman (1902 p. 384). 



656. Charpentier's Sucrose Peptone 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone (Malto) 10.0 g. 



3. Ammonium phosphate 



(neutral) 0.25 g. 



4. Sucrose 100.0 g. 



5. Arsenic Varying amounts 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjustment of reaction not given. 



(3) To sterile (2) add varying amounts 

 of a sterile solution of arsenic salt. 



Sterilization: Sterilize (2) at 120°C. Steril- 

 ize the arsenic solution by filtering thru 

 a candle. 



Use: To show the effect of metallic salts 

 (arsenic) on the growth of brewers' yeast. 

 The author reported no growth when 

 arsenic was present in one part in 1500. 



Reference: Charpentier (1915 p. 452). 



657. Lustig's Nitrate P«ptone Solution 



Constituents : 



1. Water 4000.0 cc. 



2. Potassium phosphate 1-0 g. 



3. MgS04 (crystals) 0.2 g. 



4. CaCl2 (fusum) 0.1 g. 



5. Invert sugar 5.0 g. 



6. Ca(N03)2 5.0 g. 



7. Peptone 1-0 S- 



8. CaCOs (puriss) 15.0 g. 



