CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



185 



Preparation: 



(1) Prepare a salt solution by dissolving 

 1.0 g. potassium phosphate, 0.2 g. 

 MgS04 (crystals) and 0.1 g. CaCla 

 (fusum) in 1000.0 cc. water. 



(2) Prepare an invert sugar solution by 

 dissolving 5.0 g. cane sugar in 

 1000.0 cc. water and inverting. 



(3) Dissolve 5.0 g. Ca(N03)2 in 500.0 cc. 

 water. 



(4) Mix 400.0 cc. (1), 240.0 cc. (2) and 

 240.0 cc. of (3). 



(5) Dissolve 1.0 g. peptone and 15.0 g. 

 CaCOs (puriss), in (4). 



(6) Dilute (5) to 4000.0 cc. with water. 



(7) Distribute into well sterilized 100.0 cc. 

 flasks in 30-50 cc. lots. 



Sterilization: Sterilize well on each of two 



successive days for an hour. 

 Use: To study reduction of nitrates by a 



red pigment producing organism isolated 



from water, and by soil forms. 

 Reference: Lustig (1890 p. 38), Frankland 



(1889 p. 376). 



658. Stoklasa's Nitrate Peptone Solution 



Constituents : 



1. Water 2000.0 cc. 



2. Sodium phosphate 0.5 g. 



3. K2S()4 0.2 g. 



4. MgClo 0.05 g. 



5. Glucose 2.5 g. 



6. Peptone 0.5 g. 



7. CaCO., 7.5 g. 



Preparation : 



(1) Dissolve 0.5 g. sodium phosphate, 

 0.2 g. potassium sulphate and 0.05 g. 

 magnesium chloride in 500.0 cc. 

 water. 



(2) Dissolve 2.5 g. glucose in 500.0 cc. 

 water. 



(3) Mix 200.0 cc. (1) and 120.0 cc. of (2). 



(4) Dissolve 0.5 g. peptone, 7.5 g. cal- 

 cium carbonate and 5.72 g. calcium 

 nitrate in (3). 



(5) Dilute to 2 liters. 



(6) Distribute in 500.0 cc. lots. 

 Sterilization: Sterilize in the steamer for 



3 hours. 

 Use : To study nitrate reduction by Bacillus 

 megatherium, {Bacillvs Ellenbachii) or 

 "Alinit." The author reported that the 

 nitrate was reduced about 8 to 10% after 

 19 days. Using 1.0 g. of peptone + 6.0 g. 



Ca(N03)2 nitrate reduction was less. 

 When CaCOs was omitted amount of 

 nitrate reduced with 1.0 g. peptone and 

 6.0 g. Ca(N03)2 was about 10.0%. 

 Variants: Stoklasa gave the following 

 variants: 



(a) Omitted the CaCOj. 



(b) Used 1.0 g. of peptone, 6.0 g. of 

 Ca(N03)2, with or without CaCOs. 



Reference: Stoklasa (1898 p. 285). 



659. Stutzer's Nitrate Glucose Solution 



Constituents : 



1. Water 



2. Glucose 



3. Peptone 



4. KXO3 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Reaction to be slightly alkaline. 



(3) Distribute in 10.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study denitrification by B. agilis, 

 B. nitrovorus, B. Stulzeri, B. Hartlebi. 

 B. Hartlebi was the only organisms that 

 decomposed the nitrate. 



Reference: Stutzer (1901 p. 84). 



660. Duchacek's Nitrate Glucose Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Glucose 10.0 g. 



3. NaNOs 4.0 g. 



4. Peptone 10.0 g. 



5. Sodium phosphate 0.25 g. 



6. CaS04 0.2 g. 



7. CaCla 0.05 g. 



8. MgClo 0.05 g. 



9. FeP04 0.05 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 



8 and 9 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Bacillus typhi ab- 



dominalis and Bact. coli commune. 

 Reference: Duchacek (1904 p. 162). 



661. Khouvine's Cellulose Nitrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 5.0 g. 



3. KNO3 2.5 g. 



4. NaCl., 1.0 g. 



5. Peptone (pancreatic) 1-0 g. 



6. Cellulose 



