186 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2, 3, 4, 5 in 1. 



(2) Reaction is neutral. 



(3) Distribute in 5.0 cc. lots in tubes. 



(4) Add 1 square centimeter of cellulose 

 in the form of Berzelius filter paper 

 or a little cellulose precipitated after 

 having been dissolved in Schweitzer's 

 reagent to each tube. 



Sterilization: Sterilize for 15 minutes at 

 110°. 



Use: Isolation and enrichment of B. cellu- 

 lose dissolvens. 



Variants: The author added 2.0 g. glucose. 



Reference: Khouvine (1923 p. 713). 



662. Omeliansky's Cellulose Peptone 

 Solution (Khouvine) 



Constituents : 



1. Distilled water 1000.0 cc 



2. K0HPO4 10 g. 



3. MgS04 0.5 g. 



4. KNOs 3.0 g. 



5. Peptone (Witte) 1.0 g. 



6. NaCl trace 



7. CaCOs trace 



8. Cellulose 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Reaction is neutral. 



(3) Distribute in 5.0 cc. lots in tubes. 



(4) Add 1 square centimeter of cellulose 

 in the form of Berzelius filter paper 

 or a little cellulose precipitated after 

 having been dissolved in Schweitzer's 

 reagent to each tube. 



Sterilization: Sterilize for 15 minutes at 



110°. 

 Use: Isolation and enrichment of B. 



cellulose dissolvens. 

 Variants: Khouvine added 2.0 g. glucose. 

 Reference: Khouvine (1923 p. 713). 



663. Maassen's Nitrate Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Sodium phosphate (secon- 

 dary) 0.5 g 



3. NaCl 0.5 g 



4. Soda 0.5 g 



5. MgS04 1-0 g 



6. Malic acid 7.0 g 



7. Glycerin 20.0 g 



8. Sait peter 2.5 g 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 

 7 and 8 in 1. The malic acid is to be 

 neutralized by the addition of pure soda 

 solution. 



Sterilization: Not specified. 



Use: To study ammonia production and 

 denitrification. 



Reference: Maassen (1902 p. 52). 



664. Wassermann's Nitrate Peptone Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Na2HP04 3.7 g. 



3. KH2PO4 1.4 g. 



4. CaCl2 0.4 g. 



5. KCl 3.0 g. 



6. Magnesium citrate 0.1 g. 



7. Nitrate 



8. Peptone (Witte) (1.0%) .... 10.0 g. 

 Preparation: 



(1) Dissolve 2, 3, 4, 5, 6 and 8 in 1. 



(2) Add nitrate to (1). (Amount or kind 

 not specified.) 



Sterilization: Not specified. 



Use: Differentiation of hemorrhagic septi- 

 cemia bacteria on basis of nitrate reduc- 

 tion and indol production. 



Variants: The author used 1.0% of Asch- 

 mann's, Merck's or Carne, peptone in- 

 stead of Witte's. Also used 1.0% of 

 Kuhne's driisenpeptone, albumoses from 

 Witte's peptone or sodium caseinate 

 instead of Witte's peptone. 



Reference: Wassermann (1898 p. 28). 



665. Duchacek's Nitrate Tartaric Acid 

 Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Tartaric acid 10.0 g 



3. NaNOs 4.0 g 



4. Peptone 1.0 g 



5. Sodium phosphate 0.25 g 



6. CaS04 0.20 g 



7. CaCl2 0.5 g 



8. MgCl. 0.5 g 



9. FeP04 0.5 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 



(2) Neutralize (1) by the addition of 

 soda solution. 



Sterilization: Not specified. 



Use: Cultivation of Bacz7Zus typhi abdomi- 



nalis and Bad. coli commune. 

 Reference: Duchacek (1904 p. 162). 



