CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



187 



666. Hiss' Basal Asparagin Peptone 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Asparagin 5.0 g. 



4. 5.0% litmus solution 10.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Dissolve 10.0 g. of one of the added 

 nutrients in (1). 



Sterilization: Not specified. 



Use: To study fermentation of sugars by 



the dysentery group. 

 Added nutrients : The author added 10.0 g. 

 of one of the following: 



glucose sucrose 



maltose dextrin 



mannitol 

 Reference: Hiss (1904-05 p. 29). 



667. Henneberg's Basal Asparagin Peptone 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. KH2PO4 (0.3%) 3.0 g. 



3. MgS04 (0.01%) 0.1 g. 



4. Asparagin (0.3%) 3.0 g. 



5. Peptone (1.0%) 10.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 



5.0% of one of the added nutrients in 1. 

 Sterilization: Not specified. 

 Use: Determine acid production by lactic 



acid bacteria using various carbon 



sources. Author reported no growth 



using starch or dextrin. 

 Added nutrients: The author added 5.0% 



of a large variety of carbon sources. 

 Reference: Henneberg (1903 p. 7). 



668. Nicole and Refik-Bey's Asparagin 

 Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 20.0 g. 



3. NaCI 5.0 g. 



4. K2HPO4 2.0 g. 



5. Asparagin 2.0 g. 



6. Ammonium lactate 3.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of cocco bacillus causing 



goat pneumonia, and growth of the 

 diphtheria bacilli with toxin production. 

 Reference: Nicole and Refik-Bey (1896 

 p. 325). 



66Q. Thonnessen's Asparagin Peptone 

 Solution 



Constituents: 



1. Distilled water 



2. Asparagin 



3. Peptone (siccum) 



4. K2HPO4 



5. NaCl 



6. Sodium bicarbonate. . . . 



7. AIgS04 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. (The 

 commercial K2HPO4 must be neu- 

 tralized with KOH.) 



(2) Add 0.1 g. MgS04 to sterile (1). 

 Sterilization: Method not given. 

 Use: Cultivation of anthrax bacilli. 

 Variants: The author added bouillon. 



This did not increase the growth of the 

 anthrax bacillus. 

 Reference: Thonnessen (1902 p. 824). 



670. Bokorny's Asparagin Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Asparagin (0.1%) 1.0 g. 



3. Peptone (0.025%) 0.25 g. 



4. MgS04 (0.03%) 0.3 g. 



5. CaCl. trace 



6. Sucrose (10.0%) 100.0 g. 



7. KH2PO4 (0.1, 0.2, 0.5, 1.0, 2.0 or 4.0%) 

 (1.0, 2.0, 5.0, 10.0, 20.0 or 40.0 g.) 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 

 in 1. 



Sterilization: Not specified. 



Use: To study effect of metallic salts on 

 yeast. The author reported that the 

 presence of 4.0% K2HPO4 did not pro- 

 duce an inhibitory effect on the growth of 

 yeast. Na2HP04 did not inhibit the 

 development of the yeast. 



Variants: The author used 0.5, 1.0, 2.0 or 

 5.0% Na2HP04 instead of KH2PO4. 



Reference: Bokorny (1912 p. 119). 



671. Reichenbach's Lactose Peptone 

 Solution (Quantz) 



Constituents: 

 1. Water 1000.0 cc. 



