188 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2. Na2HP04 2.5 g 



3. Asparagin 4.0 g 



4. NaCl 5.0 g 



5. Peptone (Witte) 2.5 g 



6. Lactose 5.0 g 



7. Azolitmin 0.25 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) The reaction is neutral. 

 Sterilization: Not specified. 



Use: Water analysis. B. coli grew rapidly 

 in this medium, producing the maximum 

 amount of acid in 4 days. 



Reference: Quantz (1914 p. 202). 



672. Frankel's Asparagin Peptone Solution 



(Tanner) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. CaH4(P04)2-H20 2.0 g. 



3. Ammonium lactate 6.0 g. 



4. Asparagin 4.0 g. 



5. NaCl 5.0 g. 



6. N/1 NaOH 20.0 cc. 



7. Peptone (4.0%) 40.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Tube. 



Sterilization: Sterilize in Arnold. 



Use: To study sulphur metabolism of 



fluorescent bacteria, colon typhoid group 



and others. 

 Reference: Tanner (1917 p. 586). 



673. Berthelot's Tyrosine Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (Witte) 20.0 g. 



3. Glucose 20.0 g. 



4. Tyrosine 10.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Production of aromatic hydroxy acids 

 and the study of phenol production by 

 Bacillus phenologenes. 



Variants: The author used the following 

 variants to study phenol production: 



(a) 2.0 g. tyrosine in 1000.0 cc. of peptone 

 water. 



(b) 2.0 g. tyrosine and 10.0 g. glucose in 

 1000.0 cc. peptone water. 



Reference: Berthelot (1909 p. 87), (1918 

 p. 30). 



674. Tryptophane Broth (A. P. H. A.) 



Constituents: 



1. Water (Dist.) 1000.0 cc. 



2. Tryptophane 0.3 g. 



3. K2HPO4 5.0 g. 



4. Peptone 10 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Detection of indol production. The 

 Committee stated that some American 

 brands of peptone contained certain uni- 

 form amounts of tryptophane. If such 

 peptone be used it is unnecessary to add 

 the tryptophane, but use 5.0 g. peptone 

 per liter. 



References: Committee A. P. H. A. (1917 

 p. 107), (1920 p. 107), (1923 p. 107), Rogers, 

 Clark and Lubs (1918 p. 233), Tanner 

 (1919 p. 47), Giltner (1921 p. 384), Levine 

 (1921 p. 109). 



675. Frieber's Tryptophane Peptone 

 Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. NaCl (0.5%) 5.0 g. 



3. Potassium phosphate (0.2%). 2.0 g. 



4. MgS04 (0.02%) 0.2 g. 



5. Tryptophane (0.03%) 0.3 g. 



6. Peptone (Witte) (0.25%) ... 2.5 g. 

 Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use: Indol production by Bad. pyocy- 

 aneus, Staphylococcus pyogenes, Bad. 

 pneumoniae, Bac. diphtheriae, potato 

 bacillus, disciformans and glanders bacil- 

 lus. Author reported that the organ- 

 isms listed above gave positive reactions 

 for indol when using the Salkowski 

 (H2SO4 and nitrites) test. Ehrlich's test 

 gave negative indol reaction for these 

 organisms. 



Reference: Frieber (1921-22 p. 264). 



676. Harvey's Tryptophane Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Ammonium lactate 5.0 g. 



3. Na2S04 2.0 g. 



4. MgS04 0.2 g. 



5. Peptone 30.0 g. 



6. Tryptophane 1:1000 soln. 0.1, 0.3 or 

 0.5 cc. 



