CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



189 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Detection of indol production. 

 Reference: Harvey (1921-22 p. 102). 



677. Hiss' Basal Urea Peptone Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Urea 5.0 g. 



3. Peptone 10.0 g. 



4. 5.0% litmus solution 10.0 cc. 



Preparation: (1) Dissolve 2, 3, 4 and 10.0 g. 



of one of the added nutrients in 1. 

 Sterilization: Not specified. 

 Use: To study fermentation of sugars by 



the dysentery group. 

 Added nutrients: Hiss added 10.0 g. of one 

 of the following: 



glucose sucrose 



maltose dextrin 



mannitol 

 Reference: Hiss (1904-05 p. 30). 



678. Killer's Urea Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Urea 100.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Study of decomposition of urea by 

 soil forms and protozoa; production of 

 uric acid and study of nitrogen metab- 

 olism. 



Variants : 



(a) Killer diluted the medium 5 to 10 

 times for the cultivation of protozoa. 



(b) Sears used 20.0 g. peptone and 

 10.0 g. urea to study uric acid forma- 

 tion and nitrogen metabolism. 



References: Killer (1913 p. 523), Sears (1916 

 p. 132). 



679. Cunningham's Urea Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. K.2HPO4 (0.05%) 0.5 g. 



4. Urea (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1 by steaming. 



(2) Filter. 



(3) Dissolve 1.0% urea in 1. 



(4) Distribute in 50.0 cc. quantities in 

 .300.0 cc. flasks. 

 Sterilization: Sterilize intermittently. 

 Use : Urea decomposition by Bac. Pasteuri, 



M. ureae hgue/aciens and other soil forms. 

 Variants: The author added 1.0% glucose. 

 Reference: Cunningham (1924 p. 145). 



680. Ayers and Rupp's Hippurate Peptone 



Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone (Parke-Davis) 10.0 g. 



3. K2HPO4 1.5 g. 



4. Sodium hippurate 10.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjust reaction to pH = 7.2. 

 Sterilization: Method not given. 



Use: To show hydrolysis of sodium hip- 

 purate by streptococci. 

 Reference: Ayers and Rupp (1922. p. 391). 



681. Ayers and Rupp's Pepsin Peptone 



Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Park Davis) 10.0 g. 



3. Pepsin 5.0 g.. 



CaClo 0.03 g. 



5. FeCla (1.0% soln.) 1 drop 



6. Sodium hippurate 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add NaOH to give pH = 7.1. 

 Sterilization: Method not given. 



Use: To show hydrolysis of sodium hip- 

 purate by hemolytic streptococci. 

 Author reported that streptococci from 

 human origin did not show a great in- 

 crease in volatile acids with sodium hip- 

 purate. Udder types showed increase. 



Variants: The authors omitted the FeClj 

 and added 1.0 g. NaCl. They adjusted 

 the pH from 8.0 to 9.0 and studied the 

 effect of alkaline reaction upon hydrolysis 

 of sodium hippurate by hemolytic strepto- 

 cocci. They reported that if medium 

 was suitable for the growth of strepto- 

 cocci the composition or reaction had no 

 effect on the hydrolysis of sodium hip- 

 purate. 



Reference: Ayers and Rupp (1922 pp. 390, 

 393). 



