190 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



682. MacConkey's Bile Salt Peptone 

 Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Sodium taurocholate (0.5%) 5.0 g. 



3. Glucose (0.5%) 5.0 g. 



4. Peptone (2.0%,) 20.0 g. 



5. Litmus 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add a sufficient quantity of litmus to 

 give a distinct color. 



(3) Tube, and place a Dunham's fer- 

 mentation tube in each tube. 



(4) Adjustment of reaction not given. 

 Sterilization: Method not given. 



Use: Enrichment of colon-typhoid group 

 in water and sewage analysis. The 

 author reported that in this medium B. 

 coll and the Gartner group gave acid and 

 gas in 24 hours. 



Variants : 



(a) MacConkey (1905) specified the use 

 of distilled water, Witte's peptone, 

 used 5.0 g. glucose or 10.0 g. of any 

 other carbohydrate and used 5.0 cc. 

 of a 1.0% neutral red solution in- 

 stead of litmus. 



(b) MacConkey (1905) specified the use 

 of distilled water, Witte's peptone, 

 used 5.0 cc. of a 1.0% neutral red 

 solution instead of litmus, added 

 5.0 g. KI and added 10.0 g. mannitol, 

 5.0 g. glucose or 5.0 g. lactose. 



(c) MacConkey (1908) specified the use 

 of distilled water, Witte's peptone, 

 used 2.5 cc. of a 1.0% neutral red 

 solution instead of litmus, added 

 0.3 g. CaCl2 and added one of the 

 following: 



glucose 5.0 g 



lactose 10.0 g 



sucrose 10.0 g 



dulcitol 5.0 g 



adonitol 5.0 g 



inulin 5.0 g 



If tap water be used in the above 

 solution omit the CaCl2. 



(d) Roddy (1917) used 2.5 cc. of a 1.0%, 

 neutral red solution and added 0.5% 

 glucose, dulcitol or adonitol or 1.0% 

 of any other sugar. 



(e) Levine (1921) used 5.0 g. lactose 

 instead of 5.0 g. glucose. 



(f) Levine (1921) omitted the litmus, 

 used 1.0% peptone, used ox bile or a 

 1.0% solution of dry ox bile instead 

 of sodium taurocholate and used 

 1.0% lactose instead of 0.5% glucose. 



(g) Giltner (1921) specified distilled 

 water, used 10.0 g. of lactose instead 

 of 5.0 g. glucose and added 20.0 cc. 

 of 2.0% azolitmin solution instead 

 of litmus. 



(h) Harvey (1921-22) used 5.0 cc. of a 

 1.0% neutral red solution instead of 

 litmus and added 50.0 cc. of a 20.0% 

 solution of any sugar instead of 

 glucose, 

 (i) Harvey (1921-22) specified distilled 

 water, used 4.0% peptone, 1.0% 

 sodium taurocholate and 1.0% glu- 

 cose instead of amounts given by 

 MacConkey. 

 (j) Harvey (1921-22) used 1.0%, peptone, 

 2.5% sodium taurocholate, omitted 

 the litmus and used 1.0% glycerol 

 instead of 0.5% glucose, 

 (k) Cunningham (1924) specified the use 

 of tap water, used 1.0% lactose 

 instead of 0.5% glucose and used 

 1.0% Andrades indicator instead of 

 litmus. 

 References: MacConkey (1901 p. 740), 

 (1905 pp. 334, 337), (1908 p. 325), Abel 

 (1912 p. 226), Roddy (1917 p. 42), Tanner 

 (1919 p. 46), Percival (1920 p. 307), 

 Levine (1921 p. 110), Giltner (1921 p. 382), 

 Harvey (1921-22 pp. 89, 90, 109), Klimmer 

 (1923 p. 214), Cunningham (1924 p. 103). 



683. Harrison and van der Leek's Bile Salt 

 Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) 



(1.0% or 2.0%) 10.0 or 20.0 g. 



3. Sodium taurocholate 

 (Commercial 0.5%,) . . 5.0 g. 



4. Aesculin (0.1%,) 1-0 g. 



5. Iron citrate (0.05%) . 0.5 g. 

 Preparation : 



(1) Steam 2, 3, 4 and 5 in 1 for 30 minutes. 



(2) Filter. 



(3) Adjustment of reaction not given. 



(4) Tube. 



Sterilization: Sterilize on 3 successive days 

 in the steamer. 



