192 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(b) The author omitted the Na2HP04 and 



used 10.0 g. of peptone instead of 



20.0 g. and added 10.0 g. of lactic acid 



in the form of sodium lactate. 



Reference: Sherman (1921 p. 129), (1921 



p. 383). 



685. Cannon's Basal Yeast Peptone 

 Solution 



Constituents : 



1. Tap water 1000.0 cc. 



2. Peptone (Armours) 10.0 g. 



3. Yeast (dried autolyzed). ... 10.0 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Boil 2, 3 and 4 in 1 for 15 minutes. 



(2) Flask and autoclave. 



(3) When cold shake well and filter until 

 clear through paper. 



(4) Add 1% of the added nutrients. 

 Sterilization : Sterilize in the Arnold steri- 

 lizer for 20 minutes on each of 3 successive 

 days. 



Use : Cultural study of aciduric bacteria. 



Added nutrients: The author added 1.0% 

 of any carbohydrate. The aciduric bac- 

 teria do not develop on the basic medium, 

 but when a suitable carbohydrate is 

 added the growth is luxuriant, producing 

 a pH less than 5. 



Reference: Cannon (1924 p. 229). 



686. Ayers and Madge's Basal Yeast 

 Peptone Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone (Difco) 10.0 g. 



3. Yeast (Autolized) 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Adjust to pH = 7.4. 



(3) Distribute in 50.0 cc. quantities and 

 add 0.5 cc. of one of the added nu- 

 trients to each flask. 



Sterilization: Sterilize at 15 pounds for 

 30 minutes. 



Use: To study growth promoting sub- 

 stances found in oils and fats for strepto- 

 cocci. Authors reported that sesame oil 

 and chaulmoogra oil inhibited growth, 

 while even solid paraffin stimulated 

 growth. Growth stimulation probably 

 not due to vitamin A, for mineral oils do 

 not contain this vitamin. 



Added nutrients: The author added 0.5 cc. 

 of one of the following fats or oils to each 

 50.0 cc. lot of the basic solution: 



Sesame Chia seed 



Chaulmoogra Mustard seed 



Castor Okra seed 



Corn Linseed 



Rape seed _ , fOpen kettle 



Lumbang \ Steam refined 



Cocoanut Butterfat 



p frefined Cod liver 



\ crude Mineral 1 and 2% 



Soy bean Vaseline 1 and 2% 



Olive Paraffine 1 and 2% 



Reference: Ayers and Mudge (1922 p. 458). 



687. Ayers' Basal Cerevisine Peptone 



Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Cerevisine (a dry yeast 

 preparation) 10.0 g. 



3. Peptone 10.0 g. 



Preparation: (1) Dissolve 2, 3 and 10.0 g. 



of one of the added nutrients in 1. 



Sterilization: Not specified. 



Use: To study hydrogen ion concentra- 

 tion in culture of streptococci. Author 

 reported two limiting pH zones, pH = 4.6 

 to 4.8 and pH = 5.5 to 6.0. 



Added nutrients: Ayers added 10.0 g. of 

 one of the following materials: 

 glucose raffinose 



lactose mannitol 



sucrose inulin 



Reference: Ayers (1916 p. 84). 



688. Ayers and Rupp's Yeast Infusion 



Peptone Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Dry fresh yeast 10.0 g. 



3. Peptone 10.0 g. 



Preparation : 



(1) Prepare an infusion by mi.xing 1.0% 

 of dry fresh yeast with cold distilled 

 water. Allow to stand 10 minutes. 

 Steam in Arnold for 3 minutes and 

 filter. (If filtrate not clear add 

 kieselguhr or Merck's dialysed iron 

 and filter again). 



(2) Add 1.0% peptone to yeast extract. 



(3) Adjust to pH = 7.5, heat and filter. 



(4) Tube. 



