CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



193 



Sterilization: Method not given. 



Use: General culture medium. Authors 



reported good growth of streptococci. 

 Variants : 



(a) Fresh yeast may be autolysed for 

 24 hours at 45-50 °C. and then dried 

 at low temperatures. Then add pep- 

 tone and adjust to pH = 7.4. 



(b) Dry yeast may also be autolysed and 

 then used. 



Reference: AyersandRupp (1920 p. 90-91). 



689. Ayers and Rupp's Acid Yeast Infusion 



Peptone Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Yeast (dry fresh) 10.0 g. 



3. Peptone 10.0 g. 



Preparation : 



(1) Add 10.0 g. dried fresh yeast to 

 400.0 cc. distilled HoO and 50 N/1 

 HCl. 



(2) Heat in autoclave at 14 pounds for 

 5 hour. 



(3) After heating add 50.0 cc. N/1 NaOH. 



(4) Cool and filter until clear. 



(5) Adjust to pH = 7.5. 



(6) Make up extract to 1000.0 cc. with 

 distilled water to make a 1.0% 

 solution. 



(7) Dissolve the peptone n (6). 



(8) Adjust to pH = 7.5. 



(9) Heat and filter. 

 (10) Tube. 



Sterilization: Method not given. 

 Use: General culture medium. 

 Reference: Ayers and Rupp (1920 p. 95). 



690. Ayers and Mudge's Yeast Infusion 



Peptone Solution I 



Constituents : 



1. Water 1000.0 cc. 



2. Yeast 10.0 g. 



3. Peptone (Difco) 10.0 g. 



Preparation : 



(1) Add 10.0 g. yeast to 200.0 cc. distilled 

 water, allow to stand about 1 hour, 

 shaking before filtering. 



(2) Add 50.0 g. Lloyd's Reagent (Fuller's 

 earth) and HCl to make solution 

 0.01 normal HCl. 



(3) Shake every ^ hour for four hours and 

 filter. 



(4) Make up filtrate to 1000.0 cc. 



(5) Add 1.0% Difco peptone. 



(6) Adjust to pH = 7.2. 



(7) Steam for 15 minutes. 



(8) Filter and flask. 

 Sterilization: Method not given. 



Use : To show influence of water-soluble B 

 vitamin on growth of streptococci. This 

 medium should not contain water-soluble 

 B from yeast. It gave fairly good growth, 

 about same as regular yeast extract 

 medium. 



Reference: Ayers and Mudge (1922 p. 453). 



691. Ayers and Mudge's Autolyzed Yeast 

 Peptone Solutions 



Constituents : 



1. Water 1000.0 cc. 



2. Yeast (autolized) 10.0 g. 



3. Peptone (Difco) 10.0 g. 



Preparation : 



(1) Add 10.0 g. autolized yeast to 

 500.0 cc. boiling H2O containing 

 0.01% acetic acid. 



(2) Stir for some time. 



(3) Filter and concentrate filtrate to 

 300.0 cc. at about 80°C. 



(4) To this filtrate add 95.0% alcohol 

 to get concentration of about 52.0%. 



(5) Allow to stand over night at about 

 5°C. 



(6) Filter. 



(7) Dissolve the precipitate in 1000.0 cc. 

 distilled water. 



(8) Dissolve 1.0% peptone in (6). 



(9) Adjust to pH = 7.2. 



(10) Filter. 



(11) Distribute as desired. 

 Sterilization: Method not given. 



Use: To study the effect of vitamins on the 

 growth of streptococci. The medium 

 given above should contain some soluble 

 B vitamin. Fair growth was obtained. 



Variants : The author prepared the follow- 

 ing yeast infusions and used them in- 

 stead of the solution obtained from step 

 (7) as above in preparing media, 

 (a) This medium should contain a con- 

 siderable quantity of water soluble B 

 vitamin. 



(1) Take the filtrate from step (6) above 

 and concentrate to 200.0 cc. 



(2) Add sufficient 95.0% alcohol to give 

 a 79.0% alcohol solution. 



(3) Allow to stand over night at 5°C. 



