196 



CULTURE MEDIA FOR CULTIVATIOX OF MICROORGANISMS 



Use: Cultivation of lactic acid bacteria. 

 Reference: Henneberg (1903 p. 8). 



699. Dietrich's Glycerol Yeast Infusion 

 Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) (1.0%) .... 10.0 g. 



3. Yeast (dry 1.0%) 10.0 g. 



4. Glycerol 3.0% 30.0 g. 



Preparation : 



(1) Add 1.0% dry yeast to a 1.0% Witte 

 peptone solution. 



(2) Boil 2 or 3 times in the autoclave. 



(3) Add powdered egg white or dry serum 

 to clarify. 



(4) Add 3.0% glycerol. 



(5) If the medium is not clear allow to 

 stand for several days and decant. 



Sterilization: Method not given. 



Use: Cultivation of tubercle bacilli and 



preparation of tuberculin. 

 Reference: Dietrich (1921 p. 406). 



700. Thjotta's Basal Bacterial Infusion 

 Peptone Solution 



Constituents: 



1. Peptone water 1000.0 cc. 



2. Bacterial extract 40.0 cc. 



3. Andrade indicator 

 Preparation : 



(1) Exact composition of peptone water 

 not given. 



(2) Add andrades indicator (amount not 

 given). 



(3) Dissolve 1.0% of one of the added 

 nutrients in (2). 



(4) Distribute in 5.0 cc. lots. 



(5) Suspend a 24 hour growth of B. pro- 

 teus on agar plates, in normal saline 

 solution using 1.0 cc. for each plate. 



(6) Collect in a sterile centrifuge tube. 



(7) Boil 5 minutes, centrifuge and pipette 

 off the supernatent fluid. (Reaction 

 pH = 7.6.) 



(8) Add 0.2 cc. of (7) to each tube of (4). 

 Sterilization: Not specified. 



Use: To study bacterial nutrition. All 

 sugars supported the growth of Bacillus 

 influenzae. Acid was produced (redness 

 of medium) only with glucose. Filtering 

 the extract thru a Berkefeld filter did 

 not change its value for promoting 

 growth. 



Added nutrients: The author added 1.0% 

 of one of the following materials: 

 lactose mannitol 



raffinose inulin 



maltose salicin 



glucose sucrose 



Reference: Thjotta (1921 p. 770). 



701. Wiegert's Fungus Infusion Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Fungus 250.0 g. 



3. Peptone 10.0 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Chop 250.0 g. of fungus into small 

 pieces and place in a steamer for 

 several hours. 



(2) Evaporate the fluid from the fungus 

 to a thick dark brown extract. 



(3) Add 1.0 or 2.0% of (2), 1.0% peptone 

 and 0.5% NaCl to water. 



Sterilization: Not specified. 



Use: The fungus extract is substituted for 



meat extract. 

 Reference: Wiegert (1922-23 p. 110). 



702. Will and Wanderscheck's Sulphur 



Wort Solution 



Constituents: 



1. Beer Wort 1000.0 cc. 



2. Peptone (Witte) 15.0 g. 



3. Sulphur 3.0 g. 



Preparation: (1) Add 1.5% Witte's peptone 



and 0.3% sulphur to sterile beer wort con- 

 tained in Pasteur flasks. 



Sterilization: Method not given. 



Use: Study of hydrogen sulphide produc- 

 tion by yeast. Authors reported that the 

 addition of peptone to beer wort con- 

 taining sulphur did not increase the H2S 

 production. 



Reference: Will and Wanderscheck (1906 

 p. 308). 



703. Gottheil's Mannitol Wort Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 5.0 g. 



3. Beerwort (dry) 15.0 g. 



4. Mannitol 10.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



