CULTURE MEDIA FOR CULTIVATION OF MICRO ORGAXISMS 



197 



Use: General culture medium for organ- 

 isms found in the soil, on roots and 

 rhizomes. 



Reference: Gottheil (1901 p. 432). 



704. de Kruyff's Malt Extract Peptone 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Malt extract (Merck) 7.0 g. 



3. Maltose 10.0 g. 



4. Glucose 10.0 g. 



5. Peptone 0.5 g. 



6. Asparagin 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) After sterilization add H3PO4 so that 

 100.0 cc. of the medium will neu- 

 tralize 3.0 cc. of N/1 NaOH. 



Sterilization: Method not given. 



Use: Enrichment of yeast— Saccharomyces 



javanicus. 

 Reference: de Kruyff (1908 p. 617). 



705. Zikes' Wort Peptone Solution 



Constituents: 



1. Wort 1000.0 cc. 



2. Glucose (5.0%) 50.0 g. 



3. Peptone (1.0%) 10.0 g. 



Preparation: (1) Dissolve 5.0% glucose and 



1.0% peptone in wort. 

 Sterilization: Not specified. 

 Use: Cultivation of Apiculatus yeast, 



Torula alba, Torula Molischiana, MycO' 



derma cerevisiae, Blastoderma salmoni- 



color. 

 Reference: Zikes (1911 p. 147). 



706. Peklo's Wort Peptone Solution 



Constituents: 



1. Distilled water 200.0 cc. 



2. Beer wort 800.0 cc. 



3. K2HPO4 15.0 g. 



4. K2CO3 2.0 g. 



5. MgS04 1.8 g. 



6. Peptone (Witte 0.5%) 5.0 g. 



Preparation : 



(1) Mix 1 and 2. 



(2) Dissolve 3, 4, 5 and 6 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of plant actinomyces. 

 Reference: Peklo (1910 p. 551). 



707. Otabe's Wheat Infusion Peptone 

 Solution 



Constituents: 



1. Distilled water 1600.0 cc. 



2. Wheat 1.0 pound 



3. Diastase 0.5 g. 



4. Peptone 10.0 g. 



5. NaCl 5.0 g. 



Preparation : 



(1) Roast wheat (with or without husks) 

 in an iron pan until it becomes 

 brown. 



(2) Put 1 pound of (1) into 1600.0 cc. of 

 distilled water. Do not wash the 

 wheat. 



(3) Boil in a Koch boiler for 30 minutes. 



(4) Strain thru a clean cloth. 



(5) Make up to 1000.0 cc. with distilled 

 water if the volume is below this 

 quantity. 



(6) Add 0.5 g. of taka-diastase or ordi- 

 nary diastase and shake the flask 

 well. 



(7) Keep at 30 to 40°C. for 30 minutes. 



(8) Filter. The filtrate should be quite 

 transparent with a yellowish color, 

 nearly the same as meat broth. It 

 has a sweet smell and of a slightly 

 alkaline reaction. 



(9) Add 5.0 g. NaCl and 10.0 g. peptone 

 albumin. 



(10) Boil and filter. 



(11) To prepare wheat agar add 15.0 g. 

 (in winter) and 20.0 g. (in summer) of 

 agar to the filtrate of (10). Prepare 

 almost as in the same manner as in 

 the preparation of meat agar (de- 

 tails not given). 



Sterilization: Not specified. 

 Use: Substitute for meat infusion medium. 

 May be used as a basis for special media. 

 Reference: Otabe (1919 p. 576). 



708. Ayers and Mudge's Cabbage Infusion 

 Peptone Solution 



Constituent^ : 



1. Water 1000.0 cc. 



2. Peptone (Difco) 20.0 g. 



3. Cabbage 100.0 g. 



Preparation : 



(1) Soak 100.0 g. finely minced cabbage in 

 300.0 cc. distilled water in ice box for 

 48 hours. 



