198 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(2) Steam 30 minutes and filter. 



(3) Distribute 2.0% Difco peptone solu- 

 tion into a series of flasks in 50.0 cc. 

 quantities. 



(4) Add varying amounts of filtrate of 

 (2)— 1.0 cc, 5.0 cc. and 50.0 cc. 



(5) Make each flask to 100.0 cc. volume 

 with water. 



(6) Adjust to pH = 7.2. 



(7) Steam and filter. 



(8) Tube. 



Sterilization: Method not given. 



Use: To study growth promoting sub- 

 stances for streptococci. Author re- 

 ported that the more cabbage extract 

 added, the better the growth. 



Reference: Ayers and Mudge (1922 p. 455). 



709. Kaufmann's Jequirity Seed Infusion 

 Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Jequirity seeds. 100.0 g. 



3. Peptone 20.0 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Grind 100.0 g. Jequirity seeds in a 

 mortar. 



(2) The peeled or shelled seeds now weigh 

 about 80.0 g. 



(3) Add to 1000.0 cc. of water. 



(4) Boil in a steam sterilizer for about 

 2 hours. 



(5) Cool and filter. 



(6) Dissolve peptone in (5). 



(7) Add 8 drops of concentrated soda 

 solution to each 100.0 cc. of (6). 



(8) Distribute into test tubes. 

 Sterilization: Sterilize in the usual manner 



(Method not given). 

 Use: General culture medium. Bacillus 



pyocyaneues and many other organisms 



grew very well. 

 Variants: The author gave the following 



variants: 



(a) Addition of 5.0 g. NaCl. 



(b) Addition of 60.0 g. glycerol. 

 Reference: Kaufmann (1891 p. 68). 



710. Zilces' Must Peptone Solution 



Constituents: 



1. Must 1000.0 cc. 



2. Glucose (5.0%) 50.0 g. 



3. Peptone (1.0%) 10.0 g. 



Preparation: (1) Dissolve 5.0% glucose and 



1.0% peptone in must. 

 Sterilization: Not specified. 

 Use: Cultivation of yeasts, Torula alba, 



Torula Molischiana, Mycoderma cerevisiae, 



Blastoderma salmonicolor. 

 Reference: Zikes (1911 p. 147). 



711. Henneberg's Prune Infusion Peptone 



Solution 

 Constituents : 



1. Prune infusion (1.0%) 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. Glucose (5.0%) 50.0 g. 



Preparation : 



(1) Method of preparation of 1.0% prune 

 infusion not given. 



(2) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of lactic acid bacteria. 

 Reference: Henneberg (1903 p. 8). 



712. Miquel's Wood Ash Peptone Solution 



(Besson) 

 Constituents: 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. Peptone (Chapoteaut) 20.0 g. 



4. Wood ashes 0.1 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1 by heating. 



(2) Add 0.1 g. of wood ashes to (1). 



(3) Boil. 



(4) Filter thru paper. 



(5) Add tartaric acid until the solution is 

 neutral to litmus. 



(6) Boil 5 minutes. 



(7) Filter. 



(8) Make up to 1000.0 cc. 



(9) Tube. 



Sterilization: Sterilize at 113°C. 

 Use: General culture medium. 

 Reference: Besson (1920 p. 30). 



SUBGROUP I-C. SECTION 12 



Liquid media containing peptone (or 

 other commercial digest) and at least one 

 other organic nitrogen source of animal 

 origin exclusive of extracts or infusions. 

 Ai.* Animal cells or tissues employed. 

 Bi.* Red blood cells used. 



Rivers and Kohn's Blood Cell Pep- 

 tone Solution 713 



'See As, As, Bj, B3 and B4 next page. 



