CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



199 



Wordley's Blood Cell Peptone Solu- 

 tion 714 



B2. Brain tissue used. 



Hall's Brain Peptone Solution 715 



B3. Muscular tissue (skeletal) used. 



Hall and Peterson's Meat Mash Pep- 

 tone Solution 716 



Kitasato and Weyl's Chopped Beef 



Peptone Medium 717 



B4. Other animal tissues used. 



Jablon and Pease's Liver Peptone 

 Medium 718 



Deycke's Albumin Peptone Solution. 719 



Harvey's Egg Peptone Solution 720 



Weissenbach's Albumin Peptone 

 Solution 721 



Sherman and Albus' Basal Yeast Fat 

 Peptone Solution 722 



Weiss and Wilkes-Weiss' Whole Egg 



Solution 723 



A2. Animal fluids employed. 

 Bi. Whole blood used. 



Kelser's Blood Infusion Peptone 

 Solution 724 



Wien's Blood Peptone Solution 



(Klimmer) 725 



B2. Serum used. 



Kligler's Peptone Serum Blood Me- 

 dium 726 



Hiss' Basal Serum Peptone Solution. 727 

 B3. Other serous fluids used. 



Emile-Weil's Pleuritic Serum Pep- 

 tone Solution 728 



Hiss' Basal Ascitic Fluid Peptone 

 Solution (Park, Williams and 



Krumwiede) 729 



B4. Bile used. 



Abbott's Bile Peptone Solution 730 



Stitt's Bile Peptone Solution 731 



Boudeille's Bile Peptone Solution. . . 732 



Jackson and Melia'a Bile Peptone 

 Solution 733 



Olszewski and Kohler's Bile Peptone 

 Solution 734 



Muer and Harris' Brilliant Green 

 Lactose Peptone Solution 735 



Conradi's Bile Peptone Solution 736 



A3. Animal secretions, excretions or their 



derivatives employed. 

 Bi. Milk or its derivatives used. 



Vierling's Whey Peptone Solution. . 737 



Park, Williams and Krumwiede's 

 Whey Peptone Solution 738 



Cunningham's Peptone Whey Solu- 

 tion 739 



Kan-Ichiro Morishima's Nutrose 

 Peptone Solution 740 



Elser and Huntoon's Basal Nutrose 

 Solution 741 



Loeffler's Malachite Green Nutrose 

 Solution 742 



Seliber's Basal Casein Peptone Solu- 

 tion 743 



Albus and Holm's Milk Yeast Me- 

 dium (Medium Y) 744 



B2. E.xcretions used. 



Heller's Urine Peptone Solution.... 745 



Piorkowski's Urine Peptone Solution. 746 



Khouvin and Delaunay's Cellulose 

 Fecal Infusion Solution 747 



713. Rivers and Kohn's Blood Cell Peptone 



Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (Fairchilds) 20.0 g. 



3. NaCl 5.0 g. 



4. Red blood cells 10.0 cc. 



Preparation : 



(1) Dissolve by boiling 2 and 3 in 1. 



(2) Adjust to pH = 7.4. 



(3) Add 10.0 cc. of washed blood cells 

 and heat to 95°C. (Time not given.) 



(4) Filter thru paper. 



(5) After sterilization tube or flask in 

 sterile containers and incubate to test 

 sterility. 



Sterilization: Filter thru a Mandler filter 

 to sterilize. 



Use: Cultivation of influenza bacillus caus- 

 ing meningitis. 



Variants: Added some KNO3, amount not 

 given. 



Reference: Rivers and Kohn (1921 p. 479). 



714. Wordley's Blood Cell Peptone Solution 



Constituents: 



1. Peptone water 100.0 cc. 



2. Red blood cells (Human). . . . 2.0 cc. 

 Preparation : 



(1) Exact composition of peptone water 

 not given. 



(2) Distribute in 5.0 lots. 



(3) To each tube of (2) add 0.1 cc. of 

 centrifuged human red cells. 



(4) Shake well. 

 Sterilization: Not specified. 



Use: To study haemolysis by streptococci. 

 Reference: Wordley (1921 p. 66). 



