200 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



715. Hall's Brain Peptone Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Sheep brain 1000.0 cc. 



3. Peptone 40.0 g. 



4. Dextrose. . . ^ 20.0 g. 



Preparation : 



(1) Boil sheep brains with an equal 

 volume of distilled water. 



(2) Decant water (save) and press brains 

 thru a potato ricer. 



(3) Add 2.0% peptone and 0.1% glucose 

 to the water decanted from (2). 



(4) Mix (3) and the brain tissue after it 

 has been passed thru a potato ricer. 



(5) Tube by punching thru the filling 

 funnel with a glass rod, filling tubes 

 about half full. 



Sterilization : Sterilize intermittently in the 

 Arnold sterilizer. Five daily runs of 

 30 minutes each are recommended. 



Use: To enrich anaerobes. Growth indi- 

 cated by turbidity and often gas pro- 

 duction. 



Reference: Hall (1920 p. 579). 



716. Hall and Peterson's Meat Mash 

 Peptone Medium 



Constituents : 



1. Water 1000.0 cc. 



2. Ground meat 1.0 lb. 



3. NaCl 5.0 g. 



4. Peptone (Bacto) 20.0 g. 



Preparation : 



(1) Dissolve 3 and 4 in 1. 



(2) Add 2 to (1). 



(3) Adjust to pH = 7.2 by the addition 

 of NaOH. 



(4) Tube. 



Sterilization: Sterilize in Arnold (stream- 

 ing steam) on 3 successive days. 



Use: Study of toxin production in vitro. 

 Author reported that the addition of 1.0% 

 glucose did not aid toxin production. 

 The marble seal was used to secure 

 better anaerobic conditions. 



Variants : The author added 10.0 g. glucose. 



Reference: Hall and Peterson (1923 p. 327). 



717. Kitasato and Weyl's Chopped Beef 



Peptone Medium 

 Constituents : 



1. Water 2500.0 cc. 



2. Beef 1250.0 g. 



3. Peptone 25.0 g. 



4. NaCl 10.0 g. 



Preparation : 



(1) Boil 2.5 liters of water with 1250 kg. 

 finely chopped beef, 25.0 g. peptone 

 and 10.0 g. NaCl in streaming steam. 



(2) Make slightly alkaline by the addi- 

 tion of soda. 



Sterilization: Digest in the steamer until 

 the meat extract is germ free. 



Use: Tetanin production. When the me- 

 dium has cooled following digestion, 

 inoculate and replace the air in the con- 

 tainer by hydrogen. 



Reference: Kitasato and Weyl (1890 p. 405), 



718. Jablon and Pease's Liver Peptone 

 Medium 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. Liver (rabbit, beef or human) 

 Preparation : 



(1) Dissolve 2 and 3 in 1 by boiling 

 30 minutes. 



(2) Neutralize to phenolphthalein. 



(3) Add 20.0 cc. of a normal NaOH solu- 

 tion. The reaction must be a minus 2. 



(4) Autoclave for 15 minutes at 115°. 



(5) Filter. 



(6) Tube in 10.0 cc. lots. 



(7) Add approximately 1.0 g. of human, 

 beef or rabbit liver to each tube. 



(8) Incubate 3 days to test sterility. 



(9) The final reaction should be neutral 

 or slightly alkaline. 



Sterilization: Autoclave for 15 minutes at 

 115°C. 



Use: Enrichment medium for streptococci 

 and anaerobes, from war wounds, Bacillus 

 Welchii, Bacillus oedematiens, B. sporo- 

 genes, B. putrificus and B. tetani. 



References: Jablons and Pease (1918 p. 

 1073), Harvey (1921-22 p. 97). 



719. Deycke's Albumin Peptone Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Alkaline albumin 25.0 g. 



3. NaCl 10.0 g. 



4. Peptone 10.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



