CULTURE. MEDIA FOR CULTIVATION OF MICROORGANISMS 



201 



(2) Neutralize by adding HCl drop by 

 drop, using litmus paper as an 

 indicator. 



(3) Add 2.0% of a soda solution contain- 

 ing 2 parts water to one part soda. 



(4) Filter. 



Sterilization: Method not given. 



Use: Enrichment medium for cholera 



vibrio. 

 Reference: Deycke (1893 p. 245). 



720. Harvey's Egg Peptone Solution 



Constituents: 



1. Distilled water 1100.0 cc. 



2. NaCl 5.0 g. 



3. Peptone 10.0 to 20.0 g. 



4_ Egg 100.0 cc. 



5. Na2C03 (anhydrous 



6.5%soln.) 200.0 cc. 



Preparation : 



(1) Mix the contents of whole eggs with 

 an equal volume of water. (100.0 cc. 

 egg, 100.0 cc. distilled water.) 



(2) Add an equal volume of 6.5% an- 

 hydrous NaaCOs solution to (1). 



(3) Store sterile (2) as a stock solution. 



(4) Dissolve 2 and 3 in 1 liter of distilled 

 water. 



(5) Mix 1 volume of (2) with 9 volumes 

 of (4). 



Sterilization: Sterilize (2) and (5) by heat- 

 ing for one hour at 100°C. 

 Use: Enrichment of V. cholera. 

 Reference: Harvey (1921-22 p. 86). 



721. Weissenbach's Albumin Peptone 



Solution 



Constituents: 



1. Distilled water 



2. Peptone (Chapoteaut) 4.0 g. 



3. NaCl 0.5 g. 



4. Glucose 0.2 g. 



5. Egg white 

 Preparation : 



(1) Dissolve 2 and 3 in 100.0 cc. dis- 

 tilled water. 



(2) Make slightly alkaline to litmus. 



(3) Autoclave at 120°C. for 15 minutes. 



(4) Filter and add 0.2 g. glucose to the 

 filtrate. 



(5) Add slowly one volume of egg white 

 to three volumes distilled water. 

 Shake but do not cause the mixture 

 to foam. 



(6) Add 0.5 cc. of a 10.0% soda solution 

 to each 100.0 cc. of (5). 



(7) Autoclave (6) at 115° for 15 minutes. 



(8) Filter while hot. 



(9) Mix equal volumes of (4) and (8). 

 (10) Tube in 5.0 cc. lots. 



Sterilization: Autoclave the tubes at 110°C. 



for 15 minutes. 

 Use: Cultivation of streptococci from 



wounds. 

 Reference: Weissenbach (1918 p. 491). 



722. Sherman and Albus' Basal Yeast Fat 

 Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. Yeast (dry) (1.0%) 10.0 g. 



4. Butterfat (1.0%) 10.0 g. 



5. Agar (0.1%) 1-0 g- 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 1.0%, of one of 

 the added nutrients in 1. 



(2) Adjust to pH from 6.5 to 7.0. 

 Sterilization: Not specified. 



Use: Cultivation of lactobacilli. The au- 

 thors report that the 0.1% agar is not 

 essential but seems to favor the growth 

 of some organisms. 



Added nutrients: The authors added 1.0% 

 of any desired fermentable carbohydrate. 



Variants: Lard and mineral oil were sub- 

 stituted for butterfat. 



Reference: Sherman and Albus (1922 p. 17). 



723. Weiss and Wilkes-Weiss' Egg Solution 



Constituents: 



1. Water 200.0 cc. 



2. Peptone (Difco) (1.0%) 2.0 g. 



3. Sodium phosphate (0.2%) ... 0.4 g. 



4. Agar (0.2%) 0.4 g. 



5. Eggs 2 



Preparation : 



(1) Remove the contents of two fresh 

 eggs with sterile precautions. 



(2) Dissolve 2, 3 and 4 in 1. 



(3) Emulsify (1) in (2). 



(4) Adjust to pH = 7.8 and bring slowly 

 to a boil, stirring frequently. 



(5) Tube in spirochete tubes. 

 Sterilization: Sterilize in the Arnold for 



30 minutes and seal with 1 cm. of sterile 

 yellow petrolatum while the tubes are 

 still hot. 



