204 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



medium and sterilize in the Arnold 

 for 30 minutes on each of 3 succes- 

 sive days, 

 (e) Park, Williams and Krumwiede also 



suggested the following method of 



preparation: 



(1) Dissolve 20.0 g. of peptone and 

 20.0 g. of inulin or other carbo- 

 hydrate in a liter of water. 



(2) Add sufficient litmus to give the 

 desired color. 



(3) Tube. 



(4) Sterilize in the autoclave when 

 using inulin, and on each of 3 suc- 

 cessive days when using any other 

 source of carbon. 



(5) When cool add an equal volume of 

 sterile serum to each tube. 



References: Buerger (1905 p. 524), Rue- 

 diger (1906 p. 756), Watabiki (1909 p. 366), 

 Park, Williams and Krumwiede (1924 

 p. 124). 



728. Emile-Weil's Pleuritic Serum Peptone 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl 8.0 g. 



3. Peptone 20.0 g. 



4. Glucose 6.0 g. 



5. Pleuritic serum (human) . . . 500.0 cc. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Mix one part of human pleuritic serum 

 to 2 parts (1). 



Sterilization: Not specified. 



Use: Cultivation of Bacillus leprae. 



Reference: Emile-Weil (1905 p. 798). 



729. Hiss' Basal Ascitic Fluid Peptone 



Solution (Park, Williams and 

 Krumwiede) 



Same as medium 727, Variant (e) except 

 ascitic fluid is substituted for serum. 



730. Abbott's Bile Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Bile (ox, dried) 100.0 g. 



3. Peptone 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Tube. 

 Sterilization: Not specified. 



Use: Enrichment of typhoid bacilli. 

 Reference: Abbott (1921 p. 521). 



731. Stitt's Bile Peptone Solution 

 Constituents : 



1- Bile^ 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



Preparation : 



(1) Secure ox bile from the abattoir or 

 human bile from patients undergoing 

 bladder drainage in hospitals. 



(2) Dissolve 1.0% peptone in (1). 



(3) Tube in 10.0 cc. quantities. 

 Sterilization: Method not given. 

 Use: Enrichment of typhoid bacilli. 

 Reference: Stitt (1923 p. 47). 



732. Boudeille's Bile Peptone Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Peptone 60.0 g. 



3. Glucose 



4. Bile 

 Preparation : 



(1) Dissolve 2 in 1. 



(2) Adjustment of reaction not given. 



(3) Distribute in 5.0 cc. lots in tubes. 



(4) Prepare a 5.0% glucose solution. 



(5) Add 2.0 cc. of (4) to each tube of (3). 



(6) Add various amounts of bile to each 

 tube. (0.0 to 4.0 cc. Add water to 

 other tubes to have an equal volume 

 in each tube.) 



Sterilization: Sterilize in the autoclave. 



Use : To study effect of bile on colon bacilli. 

 The presence of bile inhibited the fer- 

 mentation of glucose by the colon bacilli. 

 Enrichment medium for typhoid bacilli, 

 differentiation between streptococci, en- 

 terococcus and diplococcus. 



Variants: 



(a) Tribondeau and Dubreuil and Harvey 

 autoclaved a mixture of 1.0% glucose 

 and 1.0% peptone in beef bile at 120° 

 for 20 minutes. The solution was 

 filtered thru wet filter paper while 

 hot, tubed with fermentation tubes 

 and sterilized at 115° for 20 minutes. 

 This medium was used as an enrich- 

 ment medium for the typhoid bacilli. 

 Paratyphoid bacilli produced gas 

 while typhoid bacilli did not. 



(b) Weissenbach used the following solu- 

 tion to differentiate between strepto- 



