CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



205 



cocci, enterococci and diplococci. 

 Enterococci and diplococci clouded 

 the medium while streptococci 

 showed no growth: 



1. Water 1000.0 cc. 



2. Peptone 40.0 g. 



3. NaCl 5.0 g. 



4. Glucose 2.0 g. 



5. Bile (beef) 250.0 cc. 



References: Boudeille (1912 p. 783), Tri- 



bondeau and Dubreuil (1918 p. 131), 

 Weissenbach (1918 p. 560), Harvey (1921- 

 22 p. 89). 



733. Jackson and Melia's Bile Peptone 

 Solution 



Constituents : 



1. Bile (sterile fresh ox) 1000.0 cc. 



2. Peptone 100 g- 



3. Lactose 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Tube in fermentation tubes. 

 Sterilization: Method of sterilization of ox 



bile or final medium not given. 

 Use : Enrichment medium in water analysis. 



Intestinal forms produced gas. 

 Variants: 



(a) Obst specified that the bile should 

 never be used after one week follow- 

 ing collection. 



(b) Bunker used 20.0 to 50.0 g. of dried 

 bile in a liter of water instead of 

 1000.0 cc. of fresh bile. 



(c) Ball specified the use of a 1.0% bile 

 solution instead of fresh bile. 



(d) Ritter used a mixture of 500.0 cc. of 

 water and 500.0 cc. ox bile instead of 

 fresh ox bile. 



(e) Tanner and Heinemann specified that 

 the final medium be sterilized in the 

 Arnold sterilizer on each of 3 succes- 

 sive days. 



(f) Bacto Lactose Peptone Bile, dehy- 

 drated, is a preparation of 50 parts 

 Bacto ox Bile, 10 parts Bacto pep- 

 tone and 10 parts Bacto lactose. 

 Dissolve 70.0 g. of this preparation in 

 a liter of distilled water. Sterilize 

 in the usual manner. 



References: Jackson and Melia (1909 

 p. 194), Obst (1916 p. 76), Bunker (1916 

 pp. 85-86), Ball (1919 p. 81), Ritter (1919 

 p. 609), Tanner (1919 p. 59), Heinemann 



(1922 p. 35), Park, Williams and Krum- 

 wiede (1924 p. 132), Digestive Ferments 

 Co. (1925 p. 13). 



734. Olszewski and Kohler's Bile Peptone 

 Solution 



Constituents: 



1. Water 12,000.0 cc. 



2. Peptone 75.0 g. 



3. NaCl 50.0 g. 



4. Bile 500.0 cc. 



5. Litmus solution 600.0 cc. 



6. Lactose 75.0 g. 



Preparation : 



(1) Dissolve 75.0 g. peptone and 50.0 g. 

 NaCl in 500.0 cc. of bile by boiling. 



(2) Filter. 



(3) Dissolve 75.0 g. lactose in 600.0 cc. 

 litmus solution prepared according to 

 Kubel and Tiemann with Kahlbaum's 

 litmus (method or reference not 

 given). 



(4) Boil (3) for 15 minutes. 



(5) Filter and add NaOH or soda solu- 

 tion to neutralize if necessary. 



(6) Mix sterile (2) and sterile (5) before 

 use. 



(7) Tube. 



Sterilization: Sterilize (2) and (5) sepa- 

 rately and mix. Sterilize the mixture. 

 Method of sterilization not given. 



Use : Water analysis. Presumptive test for 

 B. coli. Add 10 parts of the water under 

 investigation to 1 part of the medium. 

 B. coli turned the medium red, other 

 forms being inhibited. 



Reference: Olszewski and Kohler (1922 

 p. 307). 



735. Muer and Harris' Brilliant Green 

 Lactose Peptone Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Oxgall (dried) 50.0 g. 



3. Peptone 10.0 g. 



4. Lactose 10-0 g. 



5. Brilliant green 0.1 g. 



Preparation : 



(1) Heat 1 liter of distilled water in a 

 double boiler until the water in the 

 outer vessel boils. 



(2) Add 50.0 g. of dried ox gall and 10.0 g. 

 peptone to (1), stirring until solution 

 is complete. 



