CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



207 



Reference: Vierling (1922 p. 94), Klimmer 

 (1923 p. 209). 



738. Park, Williams and Krumwiede's 

 Whey Peptone Solution 



Constituents : 



1. Whey 1000.0 cc. 



2. Peptone (2.0%) 20.0 g. 



3. NaCl (0.5%).. 5.0 g. 



Preparation: 



(1) Allow 3.0 quarts of unpasterized 

 milk to stand over night. 



(2) Remove the cream the next morning. 



(3) Heat the milk over a flame and stir 

 until ready to boil. 



(4) Add 6.0 cc. of glacial acetic acid 

 (0.2%) to coagulate the casein. 



(5) Strain thru cheese cloth. 



(6) Add 2.0% peptone and 0.5% NaCl. 



(7) Boil, stirring to dissolve the pep- 

 tone and salt. 



(8) Adjust the reaction to 0.5% acid to 

 phenolphthalein. 



(9) Cool to 45°C. 



(10) Add the whites of 3 eggs to clear. 



(11) Filter thru cotton and paper. 

 Sterilization: Sterilize in the autoclave at 



15 pounds for 30 minutes. 

 Use: General culture medium. 

 Reference : Park, Williams and Krumwiede 



(1924 p. 134). 



739. Cunningham's Peptone Whey Solution 



Constituents: 



1. Whey 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. NaCl (0.5%) 5.0 g. 



4. Andrades indicator (1.0%) . . 10.0 cc. 

 Preparation : 



(1) Warm 2 liters of clean, fresh skim 

 milk in a large pot at 37°C. 



(2) Add sufficient rennet to curdle. 



(3) Allow to settle for 10 minutes. 



(4) Break the curd into large pieces by 

 means of a stirring rod. 



(5) Heat to 80°C. to contract the clot 

 and to express the whey. 



(6) Strain thru a cheese cloth. 



(7) Add 1.0% peptone and 0.5% NaCl. 



(8) Steam for 30 minutes. 



(9) Neutralize to turmeric paper. 



(10) Steam for one hour. 



(11) Filter thru paper until clear. 



(12) Add 1.0% Andrades indicator. 



Sterilization: Sterilize intermittently in 



steam. 

 Use : Bacteriologic examination of milk and 



dairy products. 

 Reference: Cunningham (1924 p. 101). 



740. Kan-Ichiro Morishima's Nutrose 

 Peptone Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Nutrose 2.5 g. 



3. Peptone 10.0 g. 



4. NaCl.'. 5.0 g. 



Preparation: 



(1) Dissolve 2 in 1. 



(2) Inoculate with a culture of B. coli. 



(3) Incubate for 24 hours at 37°C. 



(4) Autoclave. 



(5) Filter. 



(6) Dissolve 3 and 4 in the filtrate. 



(7) Adjust to pH = 7.0 to 7.1. 

 Sterilization: Autoclave at 15 pounds for 



15 minutes. 

 Use : Cultivation of typhoid bacilli. 

 Reference: Kan-Ichiro Morishima (1921 



p. 277). 



741. Elser and Hun toon's Basal Nutrose 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Nutrose 10.0 g. 



3. Peptone (Witte) 10.0 g. 



4. NaCl 5.0 g. 



5. Litmus solution 



(Merck's) 5.0 to 7.5 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 5.0 to 7.5 cc. of a watery solution 

 of Merck's highly sensitized litmus. 



(3) Prepare 100.0 cc. of a 10.0% solution 

 of one of the added nutrients in dis- 

 tilled water. 



(4) Mix sterile (2) with sterile (3). 



(5) Tube in sterile tubes. 



(6) Incubate for 3 days to detect acci- 

 dental contamination. 



Sterilization: Sterilize (2) in the usual 

 manner (method not given). Sterilize 

 (3) at 100°C. for 10 minutes. 



Use: To study fermentation by meningo- 

 cocci. Other investigators used similar 

 media for the cultivation of diphtheria 

 bacilli. 



