208 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Added nutrients : 



(a) The author added 1.0% of one of the 

 following: 



glucose sucrose 



galactose mannitol 



levulose dulcitol 



lactose inulin 



maltose dextrin 



The following solutions have been 

 described as Thiel's Nutrose solu- 

 tions and were used to study fer- 

 mentation ability of diphtheria and 

 pseudo diphtheria bacilli. 



(b) Roux and Rochaix used the basic 

 solution with 50.0 cc. of Kahlbaum's 

 litmus solution instead of 5.0 to 7.5 cc. 

 Merck's without any additions. 



(c) V. Przewaski used the same basic 

 solution with 5.0 cc. of Kahlbaum's 

 litmus solution and added 20.0 cc. 

 of a 1.0% crystalline soda solution. 

 To this basic solution he added 1.0% 

 of one of the following: 



glucose inulin 



lactose mannitol 



levulose dulcitol 



mannose 

 References: Elser and Huntoon (1909 

 p. 404), Roux and Rochaix (1911 p. 247), 

 V. Przewaski (1912 p. 13), Klimmer (1923 

 p. 222). 



742. Loeffler's Malachite Green Nutrose 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone (2.0%).... 20.0 g. 



3. Nutrose (1.0%).... 10.0 g. 



4. Lactose (5.0%) .... 50.0 g. 



5. Malachite green 



(2.0% solution) .... 30.0 to 100.0 cc. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 10.6 cc. of normal NaOH and 

 cool to about 50°C. 



(3) Add from 30.0 to 100.0 cc. of a 2.0% 

 malachite green solution. 



Sterilization: Not given. 



Use: Enrichment of colon typhoid group. 



Variants : 



(a) The author added 1.0% glucose. 



(b) Klimmer prepared the medium as 

 follows : 



(1) Dissolve the peptone and glucose 

 (0.0 or 1.0%) in 800.0 cc. of dis- 

 tilled water. 



(2) Add 10.6 cc. of normal KOH. 



(3) Dissolve 10.0 g. nutrose in 200.0 cc. 

 of hot distilled water and add the 

 lactose. 



(4) Add (3) to (2). 



(5) Flask in 100.0 cc. quantities. 



(6) Sterilize on each of 3 successive days 

 for 10 minutes in streaming steam. 



(7) Add 3.0 cc. of a 2.0% solution of 

 120 Hochst malachite green to each 

 100.0 cc. lot. 



(8) Tube in 3.0 or 4.0 cc. quantities, 

 (c) Klimmer prepared the medium as in 



variant (b) above, but omitted the 

 lactose (using 1.0% glucose) and only 

 added 1.0 cc. of the 2.0% malachite 

 green per 100.0 cc. of medium instead 

 of 3.0 cc. 

 References: Loeffler (1906 pp. 289-295), 

 Klimmer (1923 p. 213). 



743. Seliber's Basal Casein Peptone 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. MgS04 0.3 g. 



4. NaCl 0.1 g. 



5. CaCl2 0.1 g. 



6. Peptone 10.0 g. 



7. Casein 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Neutralize (indicator not specified) 

 and add a slight excess of NaOH. 



(3) Add 5.0 g. casein to (2). 



(4) Heat on a salt water bath, shaking 

 thoroughly to obtain a homogenous 

 mixture of casein. 



(5) Filter or decant sterile (4). 



(6) Dissolve 1.0% of any desired carbo- 

 hydrate, alcohol, etc. in (5). 



(7) Tube. 



Sterilization: Sterilize (4) at 115°C. for 

 15 minutes. Sterilize the media after 

 being tubed in the steamer. 



Use: Detection of fermentation by coagu- 

 lation of casein. 



Variants: The author added a trace of 

 FeCl3. 



