CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



209 



Added nutrients: The author added 1.0% 

 of any carbohydrate, alcohol, etc. 



References: Seliber (1914 p. 640), Harvey 

 (1921-22 p. 96). 



744. Albus and Holm's Milk Yeast Medium 

 (Medium Y) 



Constituents: 



1. Water 1000.0 cc. 



2. Yeast (1.0%) 10.0 g. 



3. Peptone (1.0%) 10.0 g. 



4. Lactose (1.0%) 10.0 g. 



5. Milk (Skim) 10.0 cc. 



6. Brom cresol purple (5.0% 

 alcoholic solution) 0.5 cc. 



7. Sodium recinolate 

 Preparation: 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Divide into four lots and to three add 

 various quantities of sodium recino- 

 late, keeping one lot as a control. 

 The surface tension was determined 

 by the weight drop method following 

 sterilization. 



Sterilization: Method not given. 



Use: To determine the effect of surface 

 tension on growth of Lactobacillus bul- 

 garicus and Lactobacillus acidophilus. 

 The authors reported that L. bulgaricus 

 was inhibited at a surface tension lower 

 than 40 dynes, while L. acidophilus ex- 

 hibited good growth in the same medium, 

 depressed to 36 dynes. 



Variants: The authors used sodium tauro- 

 cholate and sodium glycocholate instead 

 of sodium recinolate to lower the surface 

 tension. 



Reference: Albus and Holm (1926 p. 14). 



745. Heller's Urine Peptone Solution 



Constituents: 



1. Urine., 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



Preparation : 



(1) Secure human urine as near average 

 specific gravity as possible. 



(2) Make weakly alkaline with soda. 

 End point indicated by precipitation 

 of salts. 



(3) Filter. 



(4) Add peptone and NaCl. 



(5) Boil. 



(6) Filter. 



(7) Tube. 



Sterilization: Sterilize in streaming steam. 



Use: General culture medium. The author 

 reported growth of a number of molds, 

 saprophytic and parasitic bacteria. Sug- 

 gested as a substitute for beef infusion. 



Variants : 



(a) The color and some inhibitory sub- 

 stances may be removed by animal 

 charcoal. 



(b) Various carbon sources may be added 

 if desired. 



Reference: Heller (1890 p. 893). 



746. Piorkowski's Urine Peptone Solution 



Constituents: 



1. Urine 1000.0 cc. 



2. Peptone 5.0 g. 



Preparation: 



(1) Dissolve 5.0 g. of peptone in 100.0 cc. 

 urine and distribute in 100.0 cc. lots. 



(2) Steam for 15 minutes in a steamer. 



(3) Filter. 



(4) Distribute into 10.0 cc. lots. 

 Sterilization: Sterilize for 10 to 15 minutes 



on each of 2 days using the fractional 



method. 

 Use: Differentiation of Bact. coli and 



Bacillus typhi abdomin. 

 Reference: Piorkowski (1896 p. 687). 



747. Khouvine-Delaunay's Cellulose Fecal 



Infusion Solution 



Constituents: 



1. Water 850.0 cc. 



2. Fecal infusion 250.0 cc. 



3. Cellulose (Berzelius paper). 10.0 g. 



4. Peptone (pancreatic) 1.0 g. 



5. NaCl 1.0 g. 



6. K2HPO4 10 g. 



7. CaCOs 2.0 g. 



Preparation : 



(1) Thoroughly mix 2 parts fecal ma- 

 terial with 8 to 10 parts distilled 

 water. 



(2) Filter thru a Laurent filter. 



(3) Sterilize (2) for 15 minutes at 110°C. 



(4) Allow the precipitate from (3) to 

 settle and use the clear supernatant 

 fluid. 



(5) Dissolve 4, 5, 6 and 7 in 850.0 cc. of 

 waterand250.0cc.of (4). Add 10.0 g. 

 of cellulose (Berzelius paper). 



