CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



217 



(2) Strain out the meat juice and 

 make up to 1000.0 cc. with dis- 

 tilled water. 



(3) Add a 24 hour broth culture of 

 B. coli to (2). 



(4) Incubate at 37°C. for 12 to 16 

 hours — no longer. 



(5) Mix 1.0% (10.0 g.) peptone into a 

 thin paste with a little water and 

 add to (4). 



(6) Heat in the autoclave for 20 

 minutes or in the steamer for 

 one hour. 



(7) Neutralize to phenolphthalein. 



(8) Boil over a free flame for 3 to 

 5 minutes. 



(9) Add 1.0% glucose (or any other 

 desired sugar) and 10.0 cc. of a 

 0.5% solution of neutral red and 

 stir until the sugar is dissolved. 



(10) Filter until clear. 



(11) Distribute into fermentation 

 tubes. 



(12) Sterilize in the autoclave or in 

 flowing steam. 



(e) Baker prepared a Brom thymol blue 

 medium as follows: 



(1) Digest meat and water for two 

 hours. Cook. Filter thru ab- 

 sorbent cotton and sterilize in 

 autoclave at 18 pounds for 20 

 minutes. 



(2) Inoculate cold broth with Bad. 

 saccharolyte (Rivas), incubate at 

 37°C. for 48 hours. 



(3) Sterilize in Arnold for 20 minutes. 



(4) Add peptone and NaCl. 



(5) Adjust to pH = 7.0 with brom- 

 thymol blue. 



(6) Steam for 20 minutes and readjust 

 reaction. 



(7) Filter medium. 



(8) Add 12.0 cc. per liter medium of 

 0.2% alcoholic solution of brom- 

 thymol blue. Tube. 



(9) Sterilize at 18 pounds for 20 

 minutes. 



(10) Add sterile sugar solution under 



aseptic conditions. 



References: Heinemann (1915 p. 221), 



Besemer (1917 p. 179), Kan-Ichiro Mori- 



shima (1921 p. 277), Baker (1922 pp. 301, 



302), Giltner (1921 p. 380). 



750. Hopkins and Lang's Basal Veal 

 Infusion Broth 



Constituents: 



1. Veal Infusion 1000.0 cc. 



2. Peptone (Witte) 10.0 g. 



3. XaCl 5.0 g. 



Preparation : 



(1) Preparation of veal infusion not 

 specified. 



(2) Dissolve 2 and 3 in (1), 



(3) Adjust the reaction to -f-0.3. 



(4) Dissolve 1.0% of one of the added 

 nutrients in (3). 



Sterilization: Method not given. 



Use: To study fermentation. 



Variants: The author used 2.0% Witte's 

 peptone instead of 1.0%. 



Added nutrients: The author added 1.0% 

 of one of the following: 



glucose mannitol 



lactose inulin 



sucrose soluble starch 



salicin arabinose 



raffinose 



Reference: Hopkins and Lang (1914 p. 72). 



751. Harvey's Basal Carbonate Infusion 

 Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Beef 500.0 g. 



3. Peptone 10.0 g. 



4. NaCl 5.0 g. 



5. CaCOa 5.0 to 20.0 g. 



Preparation : 



(1) See Dunham's Meat Infusion Pep- 

 tone Solution variant (bb) 750 for 

 preparation of broth from water, 

 peptone and beef. 



(2) Dissolve 1.0% of one of the added 

 nutrients in (1). 



(3) Add from 5.0 g. to 20.0 g. CaCOj to 

 (2). 



Sterilization: Method not given. 



Use: To study fermentation. 



Added nutrients: The author added 10%. 



of any desired carbohydrate, alcohol, 



etc. 

 Reference: Harvey (1921-22 p. 107). 



752. Davis and Ferry's Basal Infusion Broth 

 Constituents: 



1. Water 500.0 cc. 



