220 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(b) Rogers, Clark and Davis used 5.0 g. 

 K2HPO4 instead of 5.0 g. Na2HP04. 

 They added 1.0% of one of the 

 following materials: 



glucose rafhnose 



levulose starch 



galactose inulin 



adonitol mannitol 



sucrose glycerol 



lactose salicin 



dulcitol 



(c) Evans used 5.0 g. K2HPO4 instead 

 of 5.0 g. Na2HP04 and added 1.0% 

 of any desired carbohydrate, alco- 

 hol, etc. 



(d) Sherman and Albus used 3.0 g. beef 

 extract instead of 4.0 g. and 5.0 g. 

 K2HPO4 instead of 5.0 g. Na2HP04. 

 They added 1.0% of one of the 

 following test materials: 



glucose inulin 



galactose starch 



levulose glycerol 



maltose mannitol 



lactose salicin 



sucrose dextrin 



raffinose 



They omitted the K2HPO4 when glu- 

 cose, galactose, levulose and maltose 

 were added. 

 References: Rogers, Clark and Evans 

 (1914 p. Ill), Rogers, Clark and Davis 

 (1914 p. 421), Evans (1916 p. 445), Sher- 

 man and Albus (1918 p. 162). 



757. Stern's Basal Fuchsin Sulphite Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 20.0 g. 



3. Meat extract 10.0 g. 



4. NaCl 5.0 g. 



5. Fuchsin (10.0% alcoholic solution) 



6. Na2S04 (10.0% water solution) 



7. Chrysoidine (0.25% solution) 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjust the reaction so that it is basic 

 to phenolphthalein. 



(3) Boil and filter. 



(4) Prepare a saturated alcoholic fuchsin 

 solution. Add about 10.0 g. fuchsin 

 to 100.0 g. alcohol and allow to stand 

 in the thermostat for 24 hours. Filter 

 and place in dropping glass. 



(5) Prepare a fresh 10.0% watery sodium 

 sulfite solution. 



(6) Prepare a 0.025% watery chrysoidine 

 solution. 



(7) To each 100.0 cc. of sterile (3) add 

 5 to 6 drops alcoholic fuchsin, 2.0 cc. 

 sodium sulfite solution, 0.5 to 1.0 cc. 

 chrysoidine solution and one of the 

 added nutrients. 



(8) Distribute into tubes. 

 Sterilization: Sterilize (3); method not 



given. Sterilize the tubed medium; 

 method not given. 

 Use: Differentiation of the colon-typhoid 



group. 

 Added nutrients: The author added 1.0% 

 of one of the following: 



glucose xylose 



levulose arabinose 



galactose mannitol 



maltose glycerol 



sucrose 

 Reference: Stern (1916 p. 483). 



758. Winslow, Rothberg and Parsons Basal 



Extract Broth 

 Constituents : 



1. Water 1000.0 cc. 



2. Beef extract (Bacto) 3.0 g.. 



3. Peptone (Bacto) 5.0 g. 



4. Brom cresol purple (0.04% 

 alcoholic solution 27.0 cc. 



5. Carbohydrate 5-0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 27.0 cc. of a 0.04% alcoholic solu- 

 tion of brom cresol purple. 



(3) pH = 6.7 to 6.8. 



(4) Add 0.5% of one of the nutrients to 

 sterile (3). 



Sterilization: Sterilize at 15 pounds for 15 

 minutes. 



Use: To study fermentation of carbohy- 

 drates, alcohols, etc., by bacteria. 

 Author used streptococci. 



Added nutrients: The author added 0.5% 

 of any desired carbohydrate, alcohol, etc. 



Reference: Winslow, Rothberg and Par- 

 sons (1923 p. 151). 



759. Elser and Huntoon's Basal NShrstofE 



Heyden Broth 

 Constituents : 

 1. Distilled water 1000.0 cc. 



