CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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2. Peptone (Witte) 10.0 g. 



3. Niihrstoff Heyden 10.0 g. 



4. NaCl 5.0 g. 



5. Litmus solution 



(Merck) 5.0 to 7.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 5.0 to 7.5 cc. of a watery solution 

 of Merck's highly sensitized litmus. 



(3) Prepare 100.0 cc. of a 10.0% solution 

 of one of the added nutrients in dis- 

 tilled water. 



(4) Mix sterile (2) and sterile (3). 



(5) Tube. 



(6) Incubate for 3 days to detect acci- 

 dental contamination. 



Sterilization: Sterilize (2) in the usual 

 manner (method not given). Sterilize 

 (3) at 100°C. for 10 minutes. 

 Use: To study fermentation of carbo- 

 hydrates, alcohols, etc. by bacteria. The 

 author used meningococci. 

 Added nutrients: The authors added 1.0% 

 of one of the following: 



glucose maltose 



sucrose mannitol 



galactose dulcitol 



levulose inulin 



lactose dextrin 



Reference: Elser and Huntoon (1909 

 p. 404). 



760. Stutzer's Basal Nitrate Extract Broth 



Constituents : 



1. Water 1000.0 cc. 



2. Meat extract 5.0 g. 



3. Peptone 5.0 g. 



4. K2HPO4 1.0 g. 



5. KNOj 2.5 to 3.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and one 



of the added nutrients in 1. 



Sterilization: Method not given. 



Use: To study denitrification. Author re- 

 ported that when glucose is used as a 

 carbon source, no decomposition of KNO3 

 takes place. If the salt of an organic acid 

 (lactic acid) is used as a carbon source the 

 KNO3 is decomposed. 



Added nutrients: The author added 10.0 g. 

 of glucose, or sodium lactate. 



Reference: Stutzer (1901 p. 82). 



761. Bacto (Dehydrated) Basal ExtractBroth 

 Constituents: 



1. Water 1000.0 cc. 



2. Beef extract (Bacto) 3.0 g. 



3. Peptone (Bacto) 5.0 g. 



Preparation : 



(1) These basic media are prepared in 

 dehydrated form and contain 5 parts 

 (0.5%) of one of the added nutrients. 

 Dissolve 13.0 g. of the dehydrated 

 medium in 1000.0 cc. of distilled 

 water. 



(2) pH = 6.6 + if sterilized for 20 minutes 

 at 15 pounds pressure. 



Sterilization : Sterilize in the usual manner. 



Use: General culture medium and with the 

 addition of carbohydrates to study fer- 

 mentation. This medium conforms to 

 the "Standard Methods 1920" formula. 



Added nutrients: 



(a) Digestive Ferments Co. prepare de- 

 hydrated glucose and lactose broth. 



(b) Digestive Ferments Co. prepare au 

 Andrade Basic Medium to which they 

 add 0.5% sucrose, lactose, mannitol, 

 maltose and glucose. This dehy- 

 drated medium is the same as the one 

 given above except that 0.025 g. 

 (0.0025%) of Difco Andrade Indicator 

 is added. 



Reference: Digestive Ferments Co. (1925 

 pp. 10, 15, 16). 



762. Thoinot and Masselin's Basal Bouillon 



Constituents: 



1. Bouillon 1000.0 cc. 



Preparation: (1) Add one of the nutrients 

 to a liter of sterile peptone bouillon. 



Sterilization: Method not given. 



Use: To study fermentation of carbohy- 

 drates, alcohols, etc. by bacteria. 



Added nutrients and variants: 



(a) The authors added from 3.0 to 4.0% 

 of glucose, lactose or glycerol to the 

 basic solution. 



(b) Roux and Rochaix added 1.0 to 2.0% 

 glucose, 2.0% lactose or 1.0 to 10.0% 

 glycerol to peptone bouillon. They 

 then added 10.0 cc. of a 5.0% neutral 

 red solution or sufficient litmus to 

 give a color. 



(c) Douglas, Fleming and Colebrook 

 added a piece of one of the following 

 to bouillon. 



potato charcoal 



bran cork 



carrot sand 



cabbage card 



