222 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



grape blotting paper 



asbestos wool khaki cloth 



cotton wool rusty nail 



lint capillary tube 



sponge 



They studied the c ultivation of wound 

 anaerobes, B. perfringens Bacillus 

 malignant oedema. The authors re- 

 ported that the tube containing the 

 blotting paper showed no growth and 

 that growth took place first in the 

 interstices of the porous material. 

 Anaerobic conditions were probably 

 produced in these places. 



(d) Roddy added 0.5 to 2.0% of any 

 desired carbohydrate, alcohol, etc., 

 to bouillon. (Added 6.0% glycerol.) 



(e) Dopter and Sacquep^e added 2.0% 

 of any desired carbohydrate, alcohol, 

 etc., to a liter of sugar free peptone 

 bouillon. 



References: Thoinot and Masselin (1902 

 p. 24), Roux and Rochaix (1911 p. 109), 

 Douglas, Fleming and Colebrook (1917 

 p. 530), Roddy (1917 p. 41), Dopter and 

 Sacquepee (1921 p. 118). 



763. Klecki's Basal Bouillon 



Constituents: 



1. Bouillon 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



Preparation : 



(1) Preparation or composition of bouil- 

 lon not given. 



(2) Dissolve 2, 3 and one of the added 

 nutrients in (1). 



(3) Distribute in Pasteur fermentation 

 tubes. 



Sterilization: Method not given. 



Use: To study fermentation by Bacillus 

 saccharobutyricus. Author reported 

 that butyric acid odor was present. Fer- 

 mentation lasts much longer in the lac- 

 tose bouillon than in the lactate medium. 

 Fermentation starts after 13 to 16 hours. 



Added nutrients: The author added 100.0 g. 

 of lactose or 100.0 g. of calcium lactate. 



Reference: Klecki (1896 p. 254). 



764. Tanner's Basal Carbonate Bouillon 



Constituents: 



(1) Bouillon 1000.0 cc. 



(2) CaC03 (2.0%) 20.0 g. 



Preparation : 



(1) Dissolve one of the added nutrients 

 in peptone bouillon. 



(2) Add 2.0% of CaCOa to (1). 

 Sterilization: Method not given. 



Use: To study fermentation of carbohy- 

 drates, alcohols, etc., by bacteria. 



Variants: Dopter and Sacquepee added a 

 small piece of CaCOs to each tube. 



Added nutrients: 



(a) The author added 1 .0% of any desired 

 carbohydrate, alcohol, etc., to the 

 basic solution. 



(b) Dopter and Sacquepee added 2.0% 

 of any desired carbohydrate, alcohol, 

 etc., to the basic solution. 



References: Tanner (1919 p. 47), Dopter 

 and Sacquepee (1921 p. 119). 



765. Wolf's Basal Casein Digest Infusion 

 Broth (Kahn) 



Constituents: 



1. Water 1000.0 cc. 



2. Beef infusion 



3. Casein 200.0 g. 



Preparation: 



(1) Add 20.0 g. anhydrous NajCOs to 

 1 liter of tap water in a 2 liter fiask. 



(2) Boil, and place on a water bath. 



(3) Add 200.0 g. casein by dusting it in 

 gradually, shaking from time to time 

 to avoid lumps. 



(4) Allow the mixture to cool and add 

 3.0 g. pancreatin and 15.0 cc. chloro- 

 form. 



(5) Incubate for 5 days at 38°C. shaking 

 vigorously each day to break up any 

 crusts that form. 



(6) Add 3.0 g. more of pancreatin and 

 incubate for 10 days longer. 



(7) At the end of the 15 days, add 

 400.0 cc. N/1 HCl. 



(8) Steam for 30 minutes and filter. 



(9) Add 120.0 cc. N/1 NaOH and adjust 

 the reaction to pH = 7.2. 



(10) Prepare beef infusion broth in the 

 usual manner (details not given). 



(11) Adjust (10) to pH = 7.0. 



(12) Add 2.0% of (9) to (11). 



(13) Seed with a strain of B. coli which 

 actively ferments dextrose. 



(14) Incubate for 24 hours. 



(15) Heat in the sterilizer for 45 minutes 

 and adjust to pH 7.2 by adding 

 N/1 NaOH. 



