CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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(16) Heat in the Arnold for 15 minutes 

 and filter. 



(17) Tube in 9.0 cc. quantities. 



(18) Prepare 10.0% solutions of one of 

 the added nutrients in the sugar free 

 broth (16). 



(19) Add 1.0 cc. of sterile (18) to each 

 sterile tube of (17). 



(20) Cap the tubes with a vaseline cap. 



(21) Heat in the Arnold for 10 minutes. 

 Sterilization: Sterilize (17) in the auto- 

 clave. Sterilize (18) by heating in the 

 Arnold at 100°C. for 10 minutes. 



Use: To study the fermentation of carbo- 

 hydrates, alcohols, etc., by bacteria. 

 Kahn used the spore bearing anaerobes. 

 Inoculation was made thru the liquid 

 vaseline by means of a Pasteur pipette, 

 and immediately immersing the tubes in 

 cold water. 

 Added nutrients: The author added 1.0% 

 of one of the following: 



glucose levulose 



galactose maltose 



sucrose lactose 



raffinose mannose 



xylose arabinose 



starch inulin 



dextrin salicin 



mannitol melezitose 



glycerol inositol 



Reference: Kahn (1922 p. 166). 



766. Torrey and Buckell's Basal Ascitic 

 Fluid Infusion Broth 



Constituents: 



1. Beef infusion 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. Ascitic fluid 200.0 cc. 



Preparation : 



(1) Method of preparation or exact com- 

 position of beef infusion not given. 



(2) Inoculate (1) with B. coli and incu- 

 bate 24 hours. Filter. 



(3) To filtrate add 2 and 3 and dissolve. 



(4) Adjust reaction to pH = 7.0. 



(5) Tube in 5.0 cc. lots. 



(6) To each tube of sterile (5) add 1.0 cc. 

 sterile ascitic fluid. The fluid stored 

 in the ice box for a long period to 

 hydrolyze the fermentable materials 

 that it might contain. 



(7) Prepare 12.0% solutions of one of the 

 added nutrients. 



(8) Add 0.5 cc. of sterile (7) to each tube 

 of (6). 



(9) Incubate 3 days at 37°C. to determine 

 sterility. 



Sterilization: Sterilize (5) in the autoclave. 

 Sterilize (8) by heating in flowing steam 

 at 100°C. for 12 minutes. 



Use: To study fermentation of carbohy- 

 drates and alcohols by bacteria. Author 

 used gonococci. 



Added nutrients : The authors added 12.0 g. 

 of one of the following materials: 

 glucose maltose 



galactose levulose 



Reference: Torrey and Buckell (1922 

 p. 142). 



767. Symmers and Wilson's Basal Ascitic 

 Fluid Extract Broth 



Constituents: 



1. Water 1000.0 cc. 



2. Meat extract (Lemco) 1-0 g. 



3. NaHCOa 1.0 g. 



4. Peptone 1-0 g. 



5. Litmus (10.0% soln.) 100.0 cc. 



6. Ascitic fluid 100.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Dissolve 1.0% of one of the added 

 nutrients to sterile (1). 



(3) Add 10.0 cc. of sterile ascitic fluid to 

 each 100.0 cc. of sterile (2). 



(4) Tube in sterile tubes. 



(5) Incubate at 37°C. to test sterility. 

 Sterilization: Sterilize (1) in the autoclave. 



Sterilize (2) by steaming on each of 3 

 successive days for 10 minutes. When 

 using levulose, arabinose and xylose heat 

 over the free flame instead of the steamer. 



Use: To study fermentation of carbohy- 

 drates, alcohols, etc., by bacteria. Au- 

 thors used meningococci. 



Added nutrients and variants: 



(a) The authors added 1.0% of any de- 

 sired carbohydrate, alcohol, etc., to 

 the basic solution. 



(b) Rosenow and Towne obtained initial 

 growth of the causative agent of 

 poliomyelitis, pleomorphic strepto- 

 cocci, using a medium prepared as 

 follows: 



